Experiment set28IT024 for Pseudomonas putida KT2440

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4-Guanidinobutyric acid nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + 4-Guanidinobutyric acid (5 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 1 genes in this experiment

For nitrogen source 4-Guanidinobutyric acid in Pseudomonas putida KT2440

For nitrogen source 4-Guanidinobutyric acid across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 1
Polyamine Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
putrescine biosynthesis I 2 2 1
L-arginine degradation XI 2 1 1
L-arginine degradation X (arginine monooxygenase pathway) 3 2 1
L-arginine degradation IX (arginine:pyruvate transaminase pathway) 4 4 1
superpathway of putrescine biosynthesis 4 3 1
L-arginine degradation VIII (arginine oxidase pathway) 4 3 1
L-arginine degradation XII 4 2 1
superpathway of polyamine biosynthesis I 8 5 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 9 1
superpathway of L-arginine and L-ornithine degradation 13 11 1
superpathway of arginine and polyamine biosynthesis 17 14 1