Experiment set28IT014 for Pseudomonas putida KT2440
Nicotinic Acid nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Glucose_noNitrogen + Nicotinic Acid (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 9 genes in this experiment
For nitrogen source Nicotinic Acid in Pseudomonas putida KT2440
For nitrogen source Nicotinic Acid across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Bile acid biosynthesis
- Arginine and proline metabolism
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- Fluorene degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| methylsalicylate degradation | 2 | 2 | 2 |
| salicylate degradation I | 1 | 1 | 1 |
| nicotinate degradation I | 6 | 6 | 5 |
| picolinate degradation | 7 | 4 | 3 |
| nicotine degradation III (VPP pathway) | 10 | 5 | 3 |
| chlorosalicylate degradation | 7 | 4 | 2 |
| superpathway of nicotinate degradation | 18 | 6 | 5 |
| nicotine degradation II (pyrrolidine pathway) | 11 | 6 | 3 |
| creatinine degradation II | 5 | 3 | 1 |
| nicotinate degradation II | 5 | 2 | 1 |
| superpathway of salicylate degradation | 7 | 7 | 1 |
| naphthalene degradation to acetyl-CoA | 12 | 3 | 1 |
| superpathway of aromatic compound degradation via 2-hydroxypentadienoate | 42 | 13 | 1 |