Experiment set28IT003 for Pseudomonas putida KT2440

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Ethanolamine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + Ethanolamine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/16/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source Ethanolamine in Pseudomonas putida KT2440

For nitrogen source Ethanolamine across organisms

SEED Subsystems

Subsystem #Specific
Ethanolamine utilization 2

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
adenosylcobalamin salvage from cobalamin 5 5 4
adenosylcobinamide-GDP salvage from cobinamide I 5 5 3
adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide 6 6 3
adenosylcobinamide-GDP salvage from cobinamide II 6 5 3
cobalamin salvage (eukaryotic) 8 4 4
superpathway of adenosylcobalamin salvage from cobinamide I 8 8 3
superpathway of adenosylcobalamin salvage from cobinamide II 9 8 3
ethanolamine utilization 5 4 1
adenosylcobalamin biosynthesis II (aerobic) 33 31 3
adenosylcobalamin biosynthesis I (anaerobic) 36 29 3