Experiment set27IT089 for Escherichia coli BW25113

Compare to:

LB in EcoFAB_3.5mL

200 most detrimental genes:

  gene name fitness t score description  
b3764 yifE +3.4 21.9 hypothetical protein (NCBI) compare
b4480 hdfR +3.3 18.8 transcriptional regulator HdfR (NCBI) compare
b2289 lrhA +3.0 24.2 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b3229 sspA +2.7 9.6 stringent starvation protein A (NCBI) compare
b3753 rbsR +2.6 19.3 DNA-binding transcriptional repressor of ribose metabolism (NCBI) compare
b2684 mprA +2.4 13.8 DNA-binding transcriptional repressor of microcin B17 synthesis and multidrug efflux (NCBI) compare
b2617 smpA +2.4 10.2 small membrane protein A (VIMSS) compare
b2512 yfgL +2.3 12.0 protein assembly complex, lipoprotein component (NCBI) compare
b0178 hlpA +2.1 6.2 periplasmic chaperone (NCBI) compare
b2572 rseA +2.0 8.3 anti-sigma factor (NCBI) compare
b2571 rseB +1.9 14.2 periplasmic negative regulator of sigmaE (NCBI) compare
b3752 rbsK +1.8 10.1 ribokinase (NCBI) compare
b1642 slyA +1.8 5.9 transcriptional regulator for cryptic hemolysin (VIMSS) compare
b2494 yfgC +1.8 12.7 predicted peptidase (NCBI) compare
b1085 b1085 +1.5 2.8 orf, hypothetical protein (VIMSS) compare
b1332 ynaJ +1.5 0.9 predicted inner membrane protein (NCBI) compare
b0145 dksA +1.5 7.3 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b0232 yafN +1.3 1.4 predicted antitoxin of the YafO-YafN toxin-antitoxin system (NCBI) compare
b0957 ompA +1.3 4.8 outer membrane protein A (3a;II*;G;d) (NCBI) compare
b4503 yafF +1.3 1.2 no description compare
b0889 lrp +1.3 5.3 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b3979 thrT +1.2 0.4 tRNA-Thr (NCBI) compare
b1542 ydfI +1.1 2.8 predicted mannonate dehydrogenase (NCBI) compare
b3404 envZ +1.0 7.6 osmolarity sensor protein (NCBI) compare
b4025 pgi +1.0 7.3 glucose-6-phosphate isomerase (NCBI) compare
b0226 dinJ +1.0 3.9 predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI) compare
b4451 ryhB +1.0 1.8 Regulatory sRNA mediating positive Fur regulon response; requires Hfq for function; global iron regulator; degraded by RNase E when bound to target (RefSeq) compare
b3643 rph +0.9 3.9 ribonuclease PH (NCBI) compare
b3096 yqjB +0.9 6.4 hypothetical protein (NCBI) compare
b3194 yrbE +0.9 6.9 predicted toluene transporter subunit: membrane component of ABC superfamily (NCBI) compare
b0053 surA +0.9 5.9 peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI) compare
b4172 hfq +0.9 1.5 RNA-binding protein Hfq (NCBI) compare
b1349 recT +0.9 3.9 Rac prophage; recombination and repair protein (NCBI) compare
b3163 nlpI +0.9 5.9 hypothetical protein (NCBI) compare
b1914 uvrY +0.9 4.0 response regulator (NCBI) compare
b2939 yqgB +0.9 1.0 predicted protein (RefSeq) compare
b1422 ydcI +0.8 5.7 putative transcriptional regulator LYSR-type (VIMSS) compare
b2183 rsuA +0.8 3.0 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b1707 ydiV +0.8 4.5 hypothetical protein (NCBI) compare
b1361 ydaW +0.8 0.6 orf, hypothetical protein (VIMSS) compare
b2741 rpoS +0.8 5.3 RNA polymerase sigma factor (NCBI) compare
b1758 b1758 +0.8 1.3 putative cytochrome oxidase (VIMSS) compare
b4685 yrbN +0.8 3.4 expressed protein (RefSeq) compare
b4450 arcZ +0.8 1.7 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3234 degQ +0.8 5.5 serine endoprotease, periplasmic (NCBI) compare
b1101 ptsG +0.8 5.8 fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI) compare
b2346 vacJ +0.8 4.2 predicted lipoprotein (NCBI) compare
b1480 sra +0.7 1.7 30S ribosomal subunit protein S22 (NCBI) compare
b1954 dsrA +0.7 1.5 regulatory antisense RNA (NCBI) compare
b2258 b2258 +0.7 1.2 putative transport/receptor protein (VIMSS) compare
b0404 yajB +0.7 3.5 hypothetical protein (NCBI) compare
b2377 yfdY +0.7 2.4 predicted inner membrane protein (NCBI) compare
b0549 ybcO +0.7 1.7 DLP12 prophage; predicted protein (NCBI) compare
b2934 cmtB +0.7 0.8 predicted mannitol-specific enzyme IIA component of PTS (NCBI) compare
b1150 ymfR +0.7 0.9 e14 prophage; predicted protein (NCBI) compare
b1600 mdtJ +0.7 1.7 multidrug efflux system transporter (NCBI) compare
b2096 gatY +0.7 3.2 tagatose-bisphosphate aldolase 1 (VIMSS) compare
b2786 barA +0.6 5.8 hybrid sensory histidine kinase, in two-component regulatory system with UvrY (NCBI) compare
b3193 yrbD +0.6 4.8 predicted ABC-type organic solvent transporter (NCBI) compare
b1400 paaY +0.6 2.2 predicted hexapeptide repeat acetyltransferase (NCBI) compare
b1743 spy +0.6 2.3 envelope stress induced periplasmic protein (NCBI) compare
b4545 ypdJ +0.6 1.1 no description compare
b0688 pgm +0.6 2.4 phosphoglucomutase (NCBI) compare
b2911 ssrS +0.6 1.6 6S regulatory RNA (NCBI) compare
b1289 ycjD +0.6 2.8 hypothetical protein (NCBI) compare
b2215 ompC +0.6 3.8 outer membrane porin protein C (NCBI) compare
b4538 yoeF +0.6 0.9 hypothetical protein (RefSeq) compare
b2945 endA +0.6 4.3 DNA-specific endonuclease I (NCBI) compare
b3466 yhhL +0.6 2.9 conserved inner membrane protein (NCBI) compare
b1971 yedY +0.6 3.2 hypothetical protein (NCBI) compare
b3192 yrbC +0.6 4.4 predicted ABC-type organic solvent transporter (NCBI) compare
b4511 ybdZ +0.6 3.0 hypothetical protein (NCBI) compare
b0828 iaaA +0.6 2.8 L-asparaginase (NCBI) compare
b1895 yecG +0.6 2.4 universal stress protein (NCBI) compare
b3022 b3022 +0.6 4.1 predicted cyanide hydratase (NCBI) compare
b1347 ydaC +0.6 0.3 Rac prophage; predicted protein (NCBI) compare
b1514 lsrC +0.6 1.6 AI2 transporter (NCBI) compare
b0870 ltaE +0.6 1.6 L-allo-threonine aldolase, PLP-dependent (NCBI) compare
b3075 ebgR +0.6 2.6 DNA-binding transcriptional repressor (NCBI) compare
b3666 uhpT +0.6 3.9 sugar phosphate antiporter (NCBI) compare
b3341 rpsG +0.6 1.1 30S ribosomal protein S7 (NCBI) compare
b2807 ygdD +0.6 3.0 conserved inner membrane protein (NCBI) compare
b0796 ybiH +0.5 2.0 putative transcriptional regulator (VIMSS) compare
b1818 manY +0.5 1.5 mannose-specific enzyme IIC component of PTS (NCBI) compare
b2292 yfbS +0.5 3.8 predicted transporter (NCBI) compare
b4596 yciZ +0.5 0.8 hypothetical protein (RefSeq) compare
b2445 yffN +0.5 2.0 CPZ-55 prophage; predicted protein (RefSeq) compare
b1806 yeaY +0.5 1.6 predicted lipoprotein (NCBI) compare
b4215 ytfI +0.5 1.3 hypothetical protein (NCBI) compare
b4078 yjcO +0.5 4.3 hypothetical protein (NCBI) compare
b3292 zntR +0.5 2.4 zinc-responsive transcriptional regulator (NCBI) compare
b1446 ydcY +0.5 1.6 hypothetical protein (NCBI) compare
b3129 sohA +0.5 1.9 predicted regulator (NCBI) compare
b0990 cspG +0.5 0.6 DNA-binding transcriptional regulator (NCBI) compare
b0453 ybaY +0.5 3.8 glycoprotein/polysaccharide metabolism (VIMSS) compare
b1821 b1821 +0.5 1.3 orf, hypothetical protein (VIMSS) compare
b2896 ygfX +0.5 2.3 hypothetical protein (NCBI) compare
b3403 pck +0.5 4.0 phosphoenolpyruvate carboxykinase (NCBI) compare
b1520 b1520 +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b3430 glgC +0.5 3.6 glucose-1-phosphate adenylyltransferase (NCBI) compare
b0607 uspG +0.5 2.8 universal stress protein UP12 (NCBI) compare
b1279 yciS +0.5 0.7 conserved inner membrane protein (NCBI) compare
b2962 yggX +0.5 2.1 hypothetical protein (NCBI) compare
b0825 mipB +0.5 2.5 putative transaldolase (VIMSS) compare
b1111 ycfQ +0.5 1.4 orf, hypothetical protein (VIMSS) compare
b3191 yrbB +0.5 3.4 orf, hypothetical protein (VIMSS) compare
b1566 flxA +0.5 2.3 Qin prophage; predicted protein (NCBI) compare
b3405 ompR +0.5 3.0 osmolarity response regulator (NCBI) compare
b1487 ddpA +0.5 2.6 D-ala-D-a la transporter subunit (NCBI) compare
b1205 ychH +0.5 1.5 predicted inner membrane protein (NCBI) compare
b2814 metZ +0.5 1.0 tRNA-Met (NCBI) compare
b2356 yfdM +0.5 1.5 orf, hypothetical protein (VIMSS) compare
b4406 yaeP +0.5 1.5 hypothetical protein (NCBI) compare
b1307 pspD +0.5 1.1 peripheral inner membrane phage-shock protein (NCBI) compare
b1661 cfa +0.5 2.9 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI) compare
b2794 yqcD +0.5 3.3 hypothetical protein (NCBI) compare
b0218 yafU +0.5 1.7 predicted inner membrane protein (RefSeq) compare
b0435 bolA +0.5 2.2 possible regulator of murein genes (VIMSS) compare
b3188 sfsB +0.5 2.6 DNA-binding transcriptional activator of maltose metabolism (NCBI) compare
b0001 thrL +0.5 0.8 thr operon leader peptide (NCBI) compare
b3658 selC +0.5 1.6 tRNA-Sec (NCBI) compare
b3071 yqjI +0.5 2.2 predicted transcriptional regulator (NCBI) compare
b1936 intG +0.4 0.7 predicted defective phage integrase (pseudogene) (RefSeq) compare
b1709 btuD +0.4 0.6 vitamin B12-transporter ATPase (NCBI) compare
b2642 yfjW +0.4 0.5 CP4-57 prophage; predicted inner membrane protein (NCBI) compare
b4631 ykgQ +0.4 1.2 no description compare
b0527 ybcI +0.4 2.2 conserved inner membrane protein (NCBI) compare
b3106 yhaK +0.4 1.5 predicted pirin-related protein (NCBI) compare
b0560 nohB +0.4 0.8 DLP12 prophage; DNA packaging protein (NCBI) compare
b0247 ykfG +0.4 0.8 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b2229 b2229 +0.4 1.8 orf, hypothetical protein (VIMSS) compare
b3484 yhhI +0.4 1.6 predicted transposase (NCBI) compare
b3765 yifB +0.4 3.4 predicted bifunctional enzyme and transcriptional regulator (RefSeq) compare
b1757 b1757 +0.4 2.3 putative thiosulfate sulfur transferase (VIMSS) compare
b1871 yecP +0.4 2.2 predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI) compare
b0830 yliB +0.4 2.7 predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI) compare
b1335 ogt +0.4 1.5 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b4533 ynfO +0.4 1.7 Qin prophage; predicted protein (NCBI) compare
b2217 rcsB +0.4 2.3 DNA-binding response regulator in two-component regulatory system with RcsC and YojN (NCBI) compare
b2006 yeeW +0.4 1.8 CP4-44 prophage; predicted protein (RefSeq) compare
b0925 ycbB +0.4 3.4 predicted carboxypeptidase (NCBI) compare
b2144 sanA +0.4 3.3 hypothetical protein (NCBI) compare
b4068 yjcH +0.4 1.5 required for glycolate transport, with actP (from data) compare
b0772 ybhC +0.4 3.2 predicted pectinesterase (NCBI) compare
b1233 ychJ +0.4 1.9 hypothetical protein (NCBI) compare
b4608 ryfC +0.4 1.4 Novel sRNA, function unknown (RefSeq) compare
b1819 manZ +0.4 2.2 mannose-specific enzyme IID component of PTS (NCBI) compare
b2674 nrdI +0.4 2.5 hypothetical protein (NCBI) compare
b3672 ivbL +0.4 1.6 ilvB operon leader peptide (NCBI) compare
b0819 ybiS +0.4 2.8 hypothetical protein (NCBI) compare
b1418 cybB +0.4 1.5 cytochrome b(561) (VIMSS) compare
b2197 ccmE +0.4 1.7 periplasmic heme chaperone (NCBI) compare
b3932 hslV +0.4 3.4 ATP-dependent protease peptidase subunit (NCBI) compare
b1595 ynfL +0.4 1.4 predicted DNA-binding transcriptional regulator (NCBI) compare
b3002 yqhA +0.4 1.4 hypothetical protein (NCBI) compare
b1847 yebF +0.4 0.7 predicted protein (RefSeq) compare
b3195 yrbF +0.4 3.0 predicted toluene transporter subunit: ATP-binding component of ABC superfamily (NCBI) compare
b1916 sdiA +0.4 2.9 DNA-binding transcriptional activator (NCBI) compare
b1337 abgB +0.4 1.3 predicted peptidase, aminobenzoyl-glutamate utilization protein (NCBI) compare
b4698 mgrR +0.4 1.9 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b0929 ompF +0.4 1.7 outer membrane porin 1a (Ia;b;F) (NCBI) compare
b2114 metG +0.4 1.1 methionyl-tRNA synthetase (NCBI) compare
b3573 yiaI +0.4 3.1 predicted hydrogenase, 4Fe-4S ferredoxin-type component (NCBI) compare
b1752 ydjZ +0.4 1.7 conserved inner membrane protein (NCBI) compare
b0487 cueR +0.4 2.0 DNA-binding transcriptional activator of copper-responsive regulon genes (NCBI) compare
b1553 ydfP +0.4 1.1 Qin prophage; conserved protein (NCBI) compare
b1159 mcrA +0.4 0.8 e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI) compare
b3425 glpE +0.4 2.4 thiosulfate sulfurtransferase (NCBI) compare
b4546 ypeB +0.4 0.7 hypothetical protein (NCBI) compare
b4644 ypjI +0.4 2.4 no description compare
b1628 rsxB +0.4 0.9 electron transport complex protein RnfB (NCBI) compare
b0388 aroL +0.4 1.7 shikimate kinase II (NCBI) compare
b2836 aas +0.4 3.0 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI) compare
b0595 entB +0.4 3.1 isochorismatase (NCBI) compare
b1958 yedI +0.4 1.8 conserved inner membrane protein (NCBI) compare
b4493 gapC +0.4 1.3 no description compare
b4594 ymgJ +0.4 1.3 hypothetical protein (RefSeq) compare
b2570 rseC +0.4 2.7 RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI) compare
b4558 yifL +0.4 2.4 predicted lipoprotein (NCBI) compare
b2154 yeiG +0.4 1.8 predicted esterase (NCBI) compare
b2795 ygdH +0.4 2.9 hypothetical protein (NCBI) compare
b0225 yafQ +0.4 1.6 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1741 ydjQ +0.4 1.7 endonuclease of nucleotide excision repair (NCBI) compare
b2262 menB +0.4 1.1 naphthoate synthase (NCBI) compare
b1615 uidC +0.4 1.9 membrane-associated protein (VIMSS) compare
b4110 yjcZ +0.4 2.4 orf, hypothetical protein (VIMSS) compare
b1513 lsrA +0.4 2.4 fused AI2 transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b2293 yfbT +0.4 1.5 putative phosphatase (VIMSS) compare
b0280 yagN +0.4 2.3 CP4-6 prophage; predicted protein (NCBI) compare
b1285 gmr +0.4 2.2 modulator of Rnase II stability (NCBI) compare
b0240 crl +0.4 2.5 DNA-binding transcriptional regulator (NCBI) compare
b2004 yeeU +0.4 1.5 CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system (NCBI) compare
b1733 chbG +0.4 1.2 hypothetical protein (NCBI) compare
b3888 yiiD +0.4 3.0 predicted acetyltransferase (NCBI) compare
b1558 cspF +0.4 1.2 Qin prophage; cold shock protein (NCBI) compare
b2156 lysP +0.4 2.5 lysine transporter (NCBI) compare
b4011 yjaA +0.4 1.3 hypothetical protein (NCBI) compare
b1326 mpaA +0.4 1.8 murein peptide amidase A (RefSeq) compare
b1261 trpB +0.4 1.2 tryptophan synthase subunit beta (NCBI) compare
b1582 ynfA +0.4 1.6 hypothetical protein (NCBI) compare


Specific Phenotypes

For 10 genes in this experiment