Experiment set27IT070 for Pseudomonas putida KT2440

L-Threonine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + L-Threonine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 7 genes in this experiment

For nitrogen source L-Threonine in Pseudomonas putida KT2440

For nitrogen source L-Threonine across organisms

SEED Subsystems

Subsystem	#Specific
Glycine and Serine Utilization	2
Glycine cleavage system	2
Histidine Degradation	2
Photorespiration (oxidative C2 cycle)	2
Ammonia assimilation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Histidine metabolism
• One carbon pool by folate
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine biosynthesis IV	1	1	1
glycine biosynthesis II	3	3	2
glycine cleavage	3	3	2
L-threonine degradation IV	2	1	1
L-histidine degradation I	4	4	1
L-histidine degradation II	5	5	1
L-histidine degradation III	6	4	1
L-histidine degradation VI	8	7	1
superpathway of L-threonine metabolism	18	12	1