Experiment set27IT066 for Pseudomonas putida KT2440

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L-Isoleucine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + L-Isoleucine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 9 genes in this experiment

For nitrogen source L-Isoleucine in Pseudomonas putida KT2440

For nitrogen source L-Isoleucine across organisms

SEED Subsystems

Subsystem #Specific
Isoleucine degradation 5
Valine degradation 5
Leucine Degradation and HMG-CoA Metabolism 3
ABC transporter branched-chain amino acid (TC 3.A.1.4.1) 2
Acetyl-CoA fermentation to Butyrate 1
Anaerobic respiratory reductases 1
Butanol Biosynthesis 1
Isobutyryl-CoA to Propionyl-CoA Module 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 2
pyruvate fermentation to butanol II (engineered) 6 4 1
pyruvate fermentation to hexanol (engineered) 11 8 1
1-butanol autotrophic biosynthesis (engineered) 27 19 1