Experiment set27IT058 for Pseudomonas putida KT2440

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L-Methionine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + L-Methionine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 14 genes in this experiment

For nitrogen source L-Methionine in Pseudomonas putida KT2440

For nitrogen source L-Methionine across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 1
Arginine and Ornithine Degradation 1
Isoleucine degradation 1
Leucine Degradation and HMG-CoA Metabolism 1
Methionine Degradation 1
Phosphate metabolism 1
Polyamine Metabolism 1
Proline, 4-hydroxyproline uptake and utilization 1
Respiratory dehydrogenases 1 1
Valine degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-methionine degradation II 3 3 3
L-proline degradation I 3 3 2
seleno-amino acid detoxification and volatilization I 2 1 1
dimethyl sulfide biosynthesis from methionine 2 1 1
2-oxoisovalerate decarboxylation to isobutanoyl-CoA 3 3 1
L-threonine degradation I 6 4 2
L-arginine degradation I (arginase pathway) 3 2 1
seleno-amino acid detoxification and volatilization III 3 2 1
L-isoleucine biosynthesis I (from threonine) 7 7 2
ethene biosynthesis II (microbes) 4 1 1
(5R)-carbapenem carboxylate biosynthesis 6 1 1
superpathway of L-isoleucine biosynthesis I 13 13 2
L-Nδ-acetylornithine biosynthesis 7 5 1
hypoglycin biosynthesis 14 4 2
L-citrulline biosynthesis 8 7 1
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of L-threonine metabolism 18 12 2
superpathway of seleno-compound metabolism 19 8 2
superpathway of L-citrulline metabolism 12 9 1