Experiment set27IT042 for Pseudomonas putida KT2440
L-Ornithine nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Glucose_noNitrogen + L-Ornithine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 8 genes in this experiment
For nitrogen source L-Ornithine in Pseudomonas putida KT2440
For nitrogen source L-Ornithine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| ABC transporter branched-chain amino acid (TC 3.A.1.4.1) | 5 |
| Arginine and Ornithine Degradation | 2 |
| Proline, 4-hydroxyproline uptake and utilization | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-proline degradation I | 3 | 3 | 2 |
| L-arginine degradation I (arginase pathway) | 3 | 2 | 1 |
| ethene biosynthesis II (microbes) | 4 | 1 | 1 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 1 |
| (5R)-carbapenem carboxylate biosynthesis | 6 | 1 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 5 | 1 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |