Experiment set27IT041 for Pseudomonas putida KT2440

Beta-alanine nitrogen source

Group: nitrogen source
Media: MOPS minimal media_Glucose_noNitrogen + Beta-alanine (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 13 genes in this experiment

For nitrogen source Beta-alanine in Pseudomonas putida KT2440

For nitrogen source Beta-alanine across organisms

SEED Subsystems

Subsystem	#Specific
Pyruvate Alanine Serine Interconversions	4
Glycine and Serine Utilization	2
Polyamine Metabolism	2
Arginine and Ornithine Degradation	1
DNA-binding regulatory proteins, strays	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Respiratory dehydrogenases 1	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine degradation
• Arginine and proline metabolism
• Propanoate metabolism
• Urea cycle and metabolism of amino groups
• Puromycin biosynthesis
• Glutamate metabolism
• Alanine and aspartate metabolism
• Geraniol degradation
• Lysine biosynthesis
• Lysine degradation
• Tyrosine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• beta-Alanine metabolism
• Nucleotide sugars metabolism
• Inositol phosphate metabolism
• alpha-Linolenic acid metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Trinitrotoluene degradation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
β-alanine degradation II	2	2	2
4-aminobutanoate degradation III	2	2	1
L-alanine degradation I	2	2	1
β-alanine degradation I	2	1	1
queuosine biosynthesis I (de novo)	4	4	1
L-arginine degradation II (AST pathway)	5	5	1
L-lysine degradation IV	5	5	1
acrylate degradation I	5	3	1
queuosine biosynthesis III (queuosine salvage)	5	3	1
propanoyl-CoA degradation II	5	3	1
L-lysine degradation X	6	5	1
β-alanine biosynthesis II	6	5	1
L-lysine degradation III	6	2	1
L-lysine degradation I	7	5	1
myo-inositol degradation I	7	1	1
2,4-dinitrotoluene degradation	7	1	1
L-valine degradation I	8	6	1
superpathway of coenzyme A biosynthesis II (plants)	10	9	1
myo-, chiro- and scyllo-inositol degradation	10	1	1
superpathway of L-lysine degradation	43	23	1