Experiment set27IT011 for Pseudomonas putida KT2440
Sodium nitrite nitrogen source
Group: nitrogen sourceMedia: MOPS minimal media_Glucose_noNitrogen + Sodium nitrite (10 mM)
Culturing: Putida_ML5_JBEI, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=200 rpm
By: Mitchell Thompson on 11/8/20
Media components: 10 mM D-Glucose, 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 8 genes in this experiment
For nitrogen source Sodium nitrite in Pseudomonas putida KT2440
For nitrogen source Sodium nitrite across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Fructose and mannose metabolism
- gamma-Hexachlorocyclohexane degradation
- Bisphenol A degradation
- N-Glycan biosynthesis
- O-Glycan biosynthesis
- High-mannose type N-glycan biosynthesis
- O-Mannosyl glycan biosynthesis
- Keratan sulfate biosynthesis
- Lipopolysaccharide biosynthesis
- Peptidoglycan biosynthesis
- Glycerolipid metabolism
- Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
- Sphingolipid metabolism
- Glycosphingolipid biosynthesis - lacto and neolacto series
- Glycosphingolipid biosynthesis - globo series
- Glycosphingolipid biosynthesis - ganglio series
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Retinol metabolism
- Limonene and pinene degradation
- Carotenoid biosynthesis - General
- Zeatin biosynthesis
- Nitrogen metabolism
- Flavonoid biosynthesis
- Anthocyanin biosynthesis
- Flavone and flavonol biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| alkylnitronates degradation | 2 | 2 | 1 |
| nitrate reduction V (assimilatory) | 2 | 1 | 1 |
| glucose degradation (oxidative) | 5 | 5 | 1 |
| glucose and glucose-1-phosphate degradation | 5 | 4 | 1 |
| L-ascorbate biosynthesis VIII (engineered pathway) | 7 | 5 | 1 |