Experiment set26IT039 for Escherichia coli BW25113

Compare to:

LB 0.5x with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

200 most important genes:

  gene name fitness t score description  
b1192 ldcA -4.3 -13.6 L,D-carboxypeptidase A (NCBI) compare
b0149 mrcB -3.2 -20.7 penicillin-binding protein 1b (NCBI) compare
b2567 rnc -2.6 -1.7 ribonuclease III (NCBI) compare
b3183 obgE -2.5 -2.5 GTPase involved in cell partioning and DNA repair (NCBI) compare
b4647 mokA -1.9 -1.3 no description compare
b1105 ycfM -1.8 -5.4 predicted outer membrane lipoprotein (NCBI) compare
b1861 ruvA -1.8 -2.3 Holliday junction DNA helicase motor protein (NCBI) compare
b3390 aroK -1.8 -3.9 shikimate kinase I (NCBI) compare
b1332 ynaJ -1.7 -1.4 predicted inner membrane protein (NCBI) compare
b3940 metL -1.6 -9.2 bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI) compare
b3162 deaD -1.6 -6.3 inducible ATP-independent RNA helicase (VIMSS) compare
b3636 rpmG -1.4 -3.2 50S ribosomal protein L33 (NCBI) compare
b4375 prfC -1.4 -4.5 peptide chain release factor 3 (NCBI) compare
b3082 ygjM -1.4 -3.2 predicted DNA-binding transcriptional regulator (NCBI) compare
b2830 nudH -1.3 -2.0 dinucleoside polyphosphate hydrolase (NCBI) compare
b3261 fis -1.3 -3.1 DNA-binding protein Fis (NCBI) compare
b1290 sapF -1.3 -2.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b4155 yjeA -1.2 -0.8 putative lysyl-tRNA synthetase (VIMSS) compare
b2853 ygeI -1.2 -0.7 hypothetical protein (NCBI) compare
b1286 rnb -1.2 -3.0 exoribonuclease II (NCBI) compare
b1850 eda -1.2 -3.4 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2576 srmB -1.2 -3.7 ATP-dependent RNA helicase (NCBI) compare
b3386 rpe -1.1 -0.8 ribulose-phosphate 3-epimerase (NCBI) compare
b1130 phoP -1.1 -1.8 DNA-binding response regulator in two-component regulatory system with PhoQ (NCBI) compare
b0617 citD -1.1 -1.6 citrate lyase, acyl carrier (gamma) subunit (NCBI) compare
b4246 pyrL -1.1 -3.8 pyrBI operon leader peptide (NCBI) compare
b4146 yjeK -1.1 -2.4 predicted lysine aminomutase (NCBI) compare
b2330 yfcB -1.1 -1.8 putative adenine-specific methylase (VIMSS) compare
b3341 rpsG -1.1 -1.7 30S ribosomal protein S7 (NCBI) compare
b3021 ygiT -1.1 -1.9 predicted DNA-binding transcriptional regulator (NCBI) compare
b0740 tolB -1.1 -5.5 translocation protein TolB precursor (NCBI) compare
b4414 tff -1.1 -1.8 unknown RNA (NCBI) compare
b4202 rpsR -1.1 -1.6 30S ribosomal protein S18 (NCBI) compare
b0114 aceE -1.1 -1.7 pyruvate dehydrogenase subunit E1 (NCBI) compare
b2363 yfdT -1.1 -2.8 CPS-53 (KpLE1) prophage; predicted protein (NCBI) compare
b0161 degP -1.0 -5.4 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b1281 pyrF -1.0 -4.6 orotidine 5'-phosphate decarboxylase (NCBI) compare
b1279 yciS -1.0 -1.6 conserved inner membrane protein (NCBI) compare
b1160 elbA -1.0 -1.1 hypothetical protein (NCBI) compare
b0739 tolA -1.0 -3.7 cell envelope integrity inner membrane protein TolA (NCBI) compare
b0920 ycbC -1.0 -4.0 conserved inner membrane protein (NCBI) compare
b3642 pyrE -0.9 -4.9 orotate phosphoribosyltransferase (NCBI) compare
b0685 ybfE -0.9 -1.8 orf, hypothetical protein (VIMSS) compare
b3778 rep -0.9 -6.1 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1291 sapD -0.9 -1.3 predicted antimicrobial peptide transporter subunit (NCBI) compare
b0888 trxB -0.9 -3.0 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b2699 recA -0.9 -1.4 recombinase A (NCBI) compare
b1860 ruvB -0.9 -1.3 Holliday junction DNA helicase B (NCBI) compare
b4239 treC -0.9 -2.0 trehalose-6-P hydrolase (NCBI) compare
b3026 qseC -0.9 -4.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b3863 polA -0.8 -1.3 DNA polymerase I (NCBI) compare
b1103 ycfF -0.8 -1.8 orf, hypothetical protein (VIMSS) compare
b1292 sapC -0.8 -1.9 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1048 mdoG -0.8 -4.2 glucan biosynthesis protein, periplasmic (NCBI) compare
b3811 xerC -0.8 -1.7 site-specific tyrosine recombinase XerC (NCBI) compare
b4171 miaA -0.8 -2.5 tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI) compare
b0200 gmhB -0.8 -1.1 hypothetical protein (NCBI) compare
b0722 sdhD -0.8 -3.3 succinate dehydrogenase cytochrome b556 small membrane subunit (NCBI) compare
b0741 pal -0.8 -2.7 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b4685 yrbN -0.8 -2.0 expressed protein (RefSeq) compare
b1348 lar -0.7 -2.1 Rac prophage; restriction alleviation protein (NCBI) compare
b3839 tatC -0.7 -4.5 TatABCE protein translocation system subunit (NCBI) compare
b4215 ytfI -0.7 -1.5 hypothetical protein (NCBI) compare
b2318 truA -0.7 -2.1 tRNA pseudouridine synthase A (NCBI) compare
b0858 ybjO -0.7 -1.9 predicted inner membrane protein (NCBI) compare
b1685 ydiH -0.7 -1.2 predicted protein (RefSeq) compare
b4234 yjgA -0.7 -3.9 hypothetical protein (NCBI) compare
b4702 mgtL -0.7 -1.7 regulatory leader peptide for mgtA (NCBI) compare
b0225 yafQ -0.7 -2.4 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b1658 purR -0.7 -3.0 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b1595 ynfL -0.6 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b1540 ydfH -0.6 -1.3 predicted DNA-binding transcriptional regulator (NCBI) compare
b0340 cynS -0.6 -2.0 cyanate hydratase (NCBI) compare
b1865 nudB -0.6 -1.0 dATP pyrophosphohydrolase (NCBI) compare
b3613 yibP -0.6 -2.6 putative membrane protein (VIMSS) compare
b1062 pyrC -0.6 -3.8 dihydroorotase (NCBI) compare
b0945 pyrD -0.6 -4.5 dihydroorotate dehydrogenase (NCBI) compare
b0183 rnhB -0.6 -2.5 ribonuclease HII (NCBI) compare
b3935 priA -0.6 -2.3 primosome assembly protein PriA (NCBI) compare
b0033 carB -0.6 -4.9 carbamoyl-phosphate synthase large subunit (NCBI) compare
b2937 speB -0.6 -3.4 agmatinase (NCBI) compare
b1088 yceD -0.6 -2.5 hypothetical protein (NCBI) compare
b4042 dgkA -0.6 -1.5 diacylglycerol kinase (NCBI) compare
b2947 gshB -0.6 -2.7 glutathione synthetase (NCBI) compare
b4313 fimE -0.6 -4.4 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b4179 vacB -0.6 -3.0 putative enzyme (VIMSS) compare
b1594 dgsA -0.6 -2.0 DNA-binding transcriptional repressor (NCBI) compare
b0044 fixX -0.6 -2.3 predicted 4Fe-4S ferredoxin-type protein (NCBI) compare
b3611 yibN -0.6 -3.2 predicted rhodanese-related sulfurtransferase (NCBI) compare
b1523 yneG -0.6 -1.5 hypothetical protein (NCBI) compare
b4163 glyV -0.5 -1.2 tRNA-Gly (NCBI) compare
b4214 cysQ -0.5 -4.6 PAPS (adenosine 3'-phosphate 5'-phosphosulfate) 3'(2'),5'-bisphosphate nucleotidase (NCBI) compare
b4035 malK -0.5 -3.2 fused maltose transport subunit, ATP-binding component of ABC superfamily/regulatory protein (NCBI) compare
b0032 carA -0.5 -3.6 carbamoyl-phosphate synthase small subunit (NCBI) compare
b0889 lrp -0.5 -1.5 DNA-binding transcriptional dual regulator, leucine-binding (NCBI) compare
b2340 sixA -0.5 -2.5 phosphohistidine phosphatase (NCBI) compare
b2516 yfgA -0.5 -3.1 hypothetical protein (NCBI) compare
b1368 ynaA -0.5 -1.6 Rac prophage; predicted tail protein (RefSeq) compare
b1293 sapB -0.5 -0.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b2614 grpE -0.5 -1.0 heat shock protein (NCBI) compare
b0976 hyaE -0.5 -2.0 protein involved in processing of HyaA and HyaB proteins (NCBI) compare
b2912 ygfA -0.5 -2.0 putative ligase (VIMSS) compare
b2939 yqgB -0.5 -1.0 predicted protein (RefSeq) compare
b1049 mdoH -0.5 -3.7 glucosyltransferase MdoH (NCBI) compare
b3405 ompR -0.5 -2.8 osmolarity response regulator (NCBI) compare
b2393 nupC -0.5 -2.9 nucleoside (except guanosine) transporter (NCBI) compare
b3816 corA -0.5 -3.7 magnesium/nickel/cobalt transporter (NCBI) compare
b2293 yfbT -0.5 -1.4 putative phosphatase (VIMSS) compare
b1504 ydeS -0.5 -2.9 predicted fimbrial-like adhesin protein (NCBI) compare
b0890 ftsK -0.5 -2.5 DNA-binding membrane protein required for chromosome resolution and partitioning (NCBI) compare
b0113 pdhR -0.5 -2.3 transcriptional regulator of pyruvate dehydrogenase complex (NCBI) compare
b0247 ykfG -0.5 -1.0 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b0237 pepD -0.5 -3.8 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b4438 cyaR -0.5 -1.3 sRNA effector of ompX mRNA instability, cAMP-induced; hfq-dependent (RefSeq) compare
b2531 iscR -0.5 -1.0 DNA-binding transcriptional repressor (NCBI) compare
b0999 cbpM -0.5 -2.1 modulator of CbpA co-chaperone (NCBI) compare
b2110 yehC -0.5 -0.6 predicted periplasmic pilin chaperone (NCBI) compare
b3741 gidA -0.5 -2.6 glucose-inhibited division protein A (NCBI) compare
b1900 araG -0.5 -2.4 fused L-arabinose transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b1676 pykF -0.5 -2.0 pyruvate kinase (NCBI) compare
b0678 nagB -0.5 -2.8 glucosamine-6-phosphate deaminase (NCBI) compare
b0949 uup -0.5 -3.4 fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI) compare
b0926 ycbK -0.5 -2.3 hypothetical protein (NCBI) compare
b1548 nohA -0.5 -1.0 Qin prophage; predicted packaging protein (RefSeq) compare
b0357 yaiN -0.5 -1.6 putative alpha helix chain (VIMSS) compare
b2258 b2258 -0.5 -0.9 putative transport/receptor protein (VIMSS) compare
b2659 b2659 -0.5 -1.4 orf, hypothetical protein (VIMSS) compare
b3442 yhhZ -0.5 -0.3 hypothetical protein (NCBI) compare
b3728 pstS -0.5 -1.8 phosphate transporter subunit (NCBI) compare
b0767 ybhE -0.5 -1.9 6-phosphogluconolactonase (NCBI) compare
b3649 rpoZ -0.5 -1.6 DNA-directed RNA polymerase subunit omega (NCBI) compare
b3396 mrcA -0.5 -4.3 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b2650 ypjC -0.5 -0.2 hypothetical protein (RefSeq) compare
b2750 cysC -0.5 -2.8 adenylylsulfate kinase (NCBI) compare
b4178 yjeB -0.5 -2.5 predicted DNA-binding transcriptional regulator (NCBI) compare
b1729 ydjN -0.4 -2.1 predicted transporter (NCBI) compare
b1644 b1644 -0.4 -1.5 putative membrane protein (VIMSS) compare
b2281 nuoI -0.4 -1.6 NADH dehydrogenase subunit I (NCBI) compare
b3781 trxA -0.4 -2.3 thioredoxin 1 (VIMSS) compare
b3237 argR -0.4 -2.3 arginine repressor (NCBI) compare
b3259 prmA -0.4 -2.1 ribosomal protein L11 methyltransferase (NCBI) compare
b0014 dnaK -0.4 -0.9 molecular chaperone DnaK (NCBI) compare
b1697 ydiQ -0.4 -1.9 hypothetical protein (NCBI) compare
b3388 damX -0.4 -3.9 hypothetical protein (NCBI) compare
b1573 ydfC -0.4 -0.9 Qin prophage; predicted protein (RefSeq) compare
b3204 ptsN -0.4 -2.0 sugar-specific enzyme IIA component of PTS (NCBI) compare
b4397 creA -0.4 -1.7 hypothetical protein (NCBI) compare
b3617 kbl -0.4 -2.8 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b4011 yjaA -0.4 -2.0 hypothetical protein (NCBI) compare
b2426 ucpA -0.4 -1.5 putative oxidoreductase (VIMSS) compare
b0688 pgm -0.4 -1.3 phosphoglucomutase (NCBI) compare
b3198 kdsC -0.4 -2.0 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (NCBI) compare
b1686 ydiI -0.4 -1.3 hypothetical protein (NCBI) compare
b1282 yciH -0.4 -2.3 orf, hypothetical protein (VIMSS) compare
b0764 modB -0.4 -2.7 molybdate ABC transporter permease protein (NCBI) compare
b4201 priB -0.4 -1.5 primosomal replication protein N (NCBI) compare
b0431 cyoB -0.4 -3.5 cytochrome o ubiquinol oxidase subunit I (NCBI) compare
b2569 lepA -0.4 -2.0 GTP-binding protein LepA (NCBI) compare
b4245 pyrB -0.4 -2.8 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1643 ydhI -0.4 -2.7 predicted inner membrane protein (NCBI) compare
b1168 b1168 -0.4 -1.8 putative proteases (VIMSS) compare
b1751 ydjY -0.4 -2.2 orf, hypothetical protein (VIMSS) compare
b1989 asnV -0.4 -1.3 tRNA-Asn (NCBI) compare
b0234 yafP -0.4 -0.7 predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI) compare
b0580 ybdJ -0.4 -1.3 predicted inner membrane protein (NCBI) compare
b3029 ygiN -0.4 -1.8 quinol monooxygenase (NCBI) compare
b1058 yceO -0.4 -0.2 hypothetical protein (NCBI) compare
b3871 bipA -0.4 -3.4 GTP-binding protein (NCBI) compare
b2479 gcvR -0.4 -2.3 transcriptional regulation of gcv operon (VIMSS) compare
b0713 ybgL -0.4 -2.2 hypothetical protein (NCBI) compare
b0961 yccF -0.4 -2.2 conserved inner membrane protein (NCBI) compare
b0738 tolR -0.4 -1.3 membrane spanning protein in TolA-TolQ-TolR complex (NCBI) compare
b3027 ygiZ -0.4 -0.2 conserved inner membrane protein (NCBI) compare
b2498 upp -0.4 -1.5 uracil phosphoribosyltransferase (VIMSS) compare
b1040 csgD -0.4 -0.7 DNA-binding transcriptional activator in two-component regulatory system (NCBI) compare
b0836 yliH -0.4 -1.8 hypothetical protein (NCBI) compare
b1273 yciN -0.4 -1.0 hypothetical protein (NCBI) compare
b2783 chpR -0.4 -1.2 antitoxin of the ChpA-ChpR toxin-antitoxin system (NCBI) compare
b0585 fes -0.4 -2.3 enterobactin/ferric enterobactin esterase (NCBI) compare
b4699 fnrS -0.4 -0.4 FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq) compare
b2289 lrhA -0.4 -2.6 DNA-binding transcriptional repressor of flagellar, motility and chemotaxis genes (NCBI) compare
b3387 dam -0.4 -3.3 DNA adenine methylase (NCBI) compare
b1452 yncE -0.4 -1.9 hypothetical protein (NCBI) compare
b2966 yqgA -0.4 -2.4 predicted inner membrane protein (NCBI) compare
b2266 elaB -0.4 -1.7 hypothetical protein (NCBI) compare
b0385 yaiC -0.4 -2.3 predicted diguanylate cyclase (NCBI) compare
b0550 rusA -0.4 -1.0 DLP12 prophage; endonuclease RUS (NCBI) compare
b0696 kdpC -0.4 -1.5 potassium-transporting ATPase subunit C (NCBI) compare
b2501 ppk -0.4 -2.8 polyphosphate kinase (NCBI) compare
b3623 rfaK -0.4 -1.2 lipopolysaccharide core biosynthesis (NCBI) compare
b1263 trpD -0.4 -1.5 bifunctional indole-3-glycerol-phosphate synthase/anthranilate phosphoribosyltransferase (NCBI) compare
b3292 zntR -0.4 -1.7 zinc-responsive transcriptional regulator (NCBI) compare
b0658 ybeX -0.4 -0.4 predicteed ion transport (NCBI) compare
b2024 hisA -0.4 -0.8 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b1613 manA -0.4 -1.1 mannose-6-phosphate isomerase (VIMSS) compare
b0051 ksgA -0.4 -1.7 dimethyladenosine transferase (NCBI) compare
b4049 dusA -0.4 -2.7 tRNA-dihydrouridine synthase A (RefSeq) compare
b2284 nuoF -0.4 -1.7 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b2414 cysK -0.4 -2.6 cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI) compare
b2774 ygcW -0.4 -1.8 putative oxidoreductase (VIMSS) compare


Specific Phenotypes

None in this experiment

For Escherichia coli BW25113 in supernatant experiments

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms