Experiment set26IT037 for Escherichia coli BW25113

LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x

Group: supernatant
Media: LB (0.5x) + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.5 x)
Culturing: Keio_ML9a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 2-May-18
Media components: 5 g/L Tryptone, 2.5 g/L Yeast Extract, 2.5 g/L Sodium Chloride (final concentrations)
Growth plate: Plate1b C1

Specific Phenotypes

For 9 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Escherichia coli BW25113

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Ribonucleotide reduction	3
Oxidative stress	1
Sulfur oxidation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Purine metabolism
• Selenoamino acid metabolism
• Glutathione metabolism
• Thiamine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine biosynthesis III	1	1	1
L-cysteine degradation IV	1	1	1
adenosine deoxyribonucleotides de novo biosynthesis II	4	4	2
guanosine deoxyribonucleotides de novo biosynthesis II	4	4	2
adenosine deoxyribonucleotides de novo biosynthesis I	2	2	1
pseudouridine degradation	2	2	1
guanosine deoxyribonucleotides de novo biosynthesis I	2	2	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	4
superpathway of adenosine nucleotides de novo biosynthesis II	7	7	2
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
superpathway of guanosine nucleotides de novo biosynthesis II	8	8	2
superpathway of L-alanine biosynthesis	4	4	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
pyrimidine deoxyribonucleotides de novo biosynthesis I	9	9	2
pyrimidine deoxyribonucleotides de novo biosynthesis III	9	8	2
superpathway of adenosine nucleotides de novo biosynthesis I	5	5	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
superpathway of guanosine nucleotides de novo biosynthesis I	6	6	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
molybdopterin biosynthesis	6	6	1
superpathway of purine nucleotides de novo biosynthesis II	26	26	4
pyrimidine deoxyribonucleotides de novo biosynthesis IV	7	6	1
pyrimidine deoxyribonucleotides biosynthesis from CTP	8	6	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	2
superpathway of thiamine diphosphate biosynthesis I	10	10	1
superpathway of purine nucleotides de novo biosynthesis I	21	21	2
tRNA-uridine 2-thiolation and selenation (bacteria)	11	7	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	4
superpathway of purine nucleotide salvage	14	13	1