Experiment set25S5 for Escherichia coli BW25113

Compare to:

1/10ON stiff

200 most important genes:

  gene name fitness t score description  
b3961 oxyR -5.1 -14.3 DNA-binding transcriptional dual regulator (NCBI) compare
b0907 serC -4.2 -8.3 phosphoserine aminotransferase (NCBI) compare
b3035 tolC -3.3 -9.1 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0088 murD -3.1 -2.8 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b0438 clpX -3.1 -5.4 ATP-dependent protease ATP-binding subunit (NCBI) compare
b0437 clpP -3.0 -2.1 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b2017 yefM -2.8 -2.1 orf, hypothetical protein (VIMSS) compare
b1293 sapB -2.7 -1.8 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3868 glnG -2.7 -9.0 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b1192 ldcA -2.7 -1.8 L,D-carboxypeptidase A (NCBI) compare
b1109 ndh -2.6 -5.1 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b3164 pnp -2.5 -3.8 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b1865 nudB -2.4 -1.6 dATP pyrophosphohydrolase (NCBI) compare
b0605 ahpC -2.4 -5.2 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b3443 yrhA -2.4 -2.9 orf, hypothetical protein (VIMSS) compare
b1175 minD -2.4 -5.5 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b1382 ynbE -2.3 -2.1 predicted lipoprotein (NCBI) compare
b2912 ygfA -2.2 -1.5 putative ligase (VIMSS) compare
b1677 lpp -2.2 -2.7 murein lipoprotein (NCBI) compare
b0683 fur -2.1 -1.4 ferric uptake regulator (NCBI) compare
b1942 fliJ -2.1 -2.9 flagellar biosynthesis chaperone (NCBI) compare
b4414 tff -2.0 -4.9 unknown RNA (NCBI) compare
b3396 mrcA -2.0 -17.0 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b3405 ompR -2.0 -2.6 osmolarity response regulator (NCBI) compare
b0386 proC -2.0 -4.1 pyrroline-5-carboxylate reductase (NCBI) compare
b0149 mrcB -2.0 -11.4 penicillin-binding protein 1b (NCBI) compare
b0912 ihfB -1.9 -4.9 integration host factor subunit beta (NCBI) compare
b0049 apaH -1.9 -2.1 diadenosinetetraphosphatase (NCBI) compare
b1920 fliY -1.8 -2.7 cystine transporter subunit (NCBI) compare
b0019 nhaA -1.8 -9.4 pH-dependent sodium/proton antiporter (NCBI) compare
b3183 obgE -1.8 -1.2 GTPase involved in cell partioning and DNA repair (NCBI) compare
b2518 ndk -1.8 -2.2 nucleoside diphosphate kinase (NCBI) compare
b3390 aroK -1.7 -2.1 shikimate kinase I (NCBI) compare
b4592 yccB -1.7 -2.5 hypothetical protein (RefSeq) compare
b1830 prc -1.7 -1.6 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b2498 upp -1.7 -2.5 uracil phosphoribosyltransferase (VIMSS) compare
b2531 iscR -1.7 -1.7 DNA-binding transcriptional repressor (NCBI) compare
b0439 lon -1.6 -3.7 DNA-binding ATP-dependent protease La (NCBI) compare
b0740 tolB -1.6 -1.9 translocation protein TolB precursor (NCBI) compare
b3790 rffC -1.6 -4.0 TDP-fucosamine acetyltransferase (RefSeq) compare
b0767 ybhE -1.6 -3.8 6-phosphogluconolactonase (NCBI) compare
b1176 minC -1.5 -2.3 septum formation inhibitor (NCBI) compare
b2910 zapA -1.4 -5.3 protein that localizes to the cytokinetic ring (NCBI) compare
b0015 dnaJ -1.4 -7.8 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1286 rnb -1.4 -1.8 exoribonuclease II (NCBI) compare
b1335 ogt -1.4 -3.4 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b3147 yraM -1.4 -9.3 hypothetical protein (NCBI) compare
b3791 rffA -1.4 -5.0 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b2808 gcvA -1.4 -3.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3781 trxA -1.4 -4.1 thioredoxin 1 (VIMSS) compare
b0222 lpcA -1.3 -2.2 phosphoheptose isomerase (NCBI) compare
b1185 dsbB -1.3 -3.8 reoxidizes DsbA protein following formation of disulfide bond in P-ring of flagella. (VIMSS) compare
b2741 rpoS -1.3 -5.4 RNA polymerase sigma factor (NCBI) compare
b3387 dam -1.3 -8.2 DNA adenine methylase (NCBI) compare
b1291 sapD -1.3 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b1148 ymfM -1.3 -3.1 e14 prophage; predicted protein (NCBI) compare
b2501 ppk -1.3 -5.4 polyphosphate kinase (NCBI) compare
b1348 lar -1.3 -1.5 Rac prophage; restriction alleviation protein (NCBI) compare
b1038 csgF -1.3 -2.2 predicted transport protein (NCBI) compare
b1070 flgN -1.3 -1.4 export chaperone for FlgK and FlgL (NCBI) compare
b1556 b1556 -1.3 -2.2 orf, hypothetical protein (VIMSS) compare
b3163 nlpI -1.2 -3.3 hypothetical protein (NCBI) compare
b0051 ksgA -1.2 -3.1 dimethyladenosine transferase (NCBI) compare
b4702 mgtL -1.2 -1.8 regulatory leader peptide for mgtA (NCBI) compare
b1105 ycfM -1.2 -3.0 predicted outer membrane lipoprotein (NCBI) compare
b4201 priB -1.2 -1.9 primosomal replication protein N (NCBI) compare
b3341 rpsG -1.2 -1.5 30S ribosomal protein S7 (NCBI) compare
b4545 ypdJ -1.2 -2.1 no description compare
b2512 yfgL -1.2 -4.2 protein assembly complex, lipoprotein component (NCBI) compare
b3764 yifE -1.2 -4.1 hypothetical protein (NCBI) compare
b3839 tatC -1.2 -4.8 TatABCE protein translocation system subunit (NCBI) compare
b0606 ahpF -1.2 -5.1 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b1698 ydiR -1.2 -3.7 predicted electron transfer flavoprotein, FAD-binding (NCBI) compare
b3650 spoT -1.1 -5.6 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b2356 yfdM -1.1 -1.6 orf, hypothetical protein (VIMSS) compare
b0082 mraW -1.1 -3.0 S-adenosyl-methyltransferase (NCBI) compare
b2215 ompC -1.1 -5.7 outer membrane porin protein C (NCBI) compare
b3162 deaD -1.1 -1.7 inducible ATP-independent RNA helicase (VIMSS) compare
b4503 yafF -1.1 -0.9 no description compare
b4313 fimE -1.1 -6.7 tyrosine recombinase/inversion of on/off regulator of fimA (NCBI) compare
b1462 b1462 -1.1 -1.2 orf, hypothetical protein (VIMSS) compare
b0741 pal -1.1 -1.8 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b0125 hpt -1.1 -4.1 hypoxanthine phosphoribosyltransferase (VIMSS) compare
b1709 btuD -1.1 -1.4 vitamin B12-transporter ATPase (NCBI) compare
b0920 ycbC -1.1 -4.1 conserved inner membrane protein (NCBI) compare
b1600 mdtJ -1.0 -1.2 multidrug efflux system transporter (NCBI) compare
b4410 ecnA -1.0 -1.4 entericidin A membrane lipoprotein, antidote entericidin B (NCBI) compare
b2287 nuoB -1.0 -3.0 NADH dehydrogenase subunit B (NCBI) compare
b2189 proL -1.0 -1.5 tRNA-Pro (NCBI) compare
b2947 gshB -1.0 -3.5 glutathione synthetase (NCBI) compare
b3928 yiiU -1.0 -4.7 hypothetical protein (NCBI) compare
b1936 intG -1.0 -0.6 predicted defective phage integrase (pseudogene) (RefSeq) compare
b3500 gor -1.0 -1.3 glutathione reductase (NCBI) compare
b4529 ydbJ -1.0 -0.9 hypothetical protein (NCBI) compare
b2620 smpB -1.0 -0.6 SsrA-binding protein (NCBI) compare
b3021 ygiT -1.0 -1.8 predicted DNA-binding transcriptional regulator (NCBI) compare
b2582 trxC -1.0 -2.1 thioredoxin 2 (NCBI) compare
b0802 ybiJ -1.0 -1.4 hypothetical protein (NCBI) compare
b2689 yqaA -1.0 -2.7 conserved inner membrane protein (NCBI) compare
b0161 degP -1.0 -5.1 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b1438 ydcQ -1.0 -1.9 predicted DNA-binding transcriptional regulator (RefSeq) compare
b3652 recG -1.0 -5.7 ATP-dependent DNA helicase (NCBI) compare
b1804 rnd -0.9 -2.9 ribonuclease D (NCBI) compare
b3169 nusA -0.9 -1.5 transcription elongation factor NusA (NCBI) compare
b4203 rplI -0.9 -3.0 50S ribosomal protein L9 (NCBI) compare
b2093 gatB -0.9 -2.7 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2953 yggU -0.9 -1.7 orf, hypothetical protein (VIMSS) compare
b3107 yhaL -0.9 -1.5 orf, hypothetical protein (VIMSS) compare
b4053 alr -0.9 -6.4 alanine racemase (NCBI) compare
b3778 rep -0.9 -3.8 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b3933 ftsN -0.9 -5.3 essential cell division protein (NCBI) compare
b4480 hdfR -0.9 -4.1 transcriptional regulator HdfR (NCBI) compare
b2659 b2659 -0.9 -1.6 orf, hypothetical protein (VIMSS) compare
b3784 rfe -0.9 -5.6 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b1103 ycfF -0.9 -1.0 orf, hypothetical protein (VIMSS) compare
b0888 trxB -0.9 -1.3 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1882 cheY -0.9 -0.4 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b2479 gcvR -0.9 -3.5 transcriptional regulation of gcv operon (VIMSS) compare
b3787 rffD -0.9 -4.8 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1074 flgC -0.8 -1.1 flagellar basal-body rod protein C (NCBI) compare
b2906 visC -0.8 -3.9 hypothetical protein (NCBI) compare
b1147 ymfL -0.8 -1.7 e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq) compare
b3414 gntY -0.8 -3.8 predicted gluconate transport associated protein (NCBI) compare
b4655 ythA -0.8 -1.0 expressed protein (RefSeq) compare
b2448 yffQ -0.8 -1.9 CPZ-55 prophage; predicted protein (RefSeq) compare
b3786 rffE -0.8 -4.9 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b3545 proK -0.8 -1.1 tRNA-Pro (NCBI) compare
b4533 ynfO -0.8 -1.4 Qin prophage; predicted protein (NCBI) compare
b0237 pepD -0.8 -4.4 aminoacyl-histidine dipeptidase (peptidase D) (NCBI) compare
b4202 rpsR -0.8 -0.5 30S ribosomal protein S18 (NCBI) compare
b4413 sokC -0.8 -1.5 regulatory antisense RNA (NCBI) compare
b4653 yicT -0.8 -1.9 no description compare
b4234 yjgA -0.8 -2.8 hypothetical protein (NCBI) compare
b3026 qseC -0.8 -0.7 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b4701 sokX -0.8 -0.7 sok-related sRNA, function unknown (RefSeq) compare
b3871 bipA -0.8 -5.8 GTP-binding protein (NCBI) compare
b0435 bolA -0.8 -4.3 possible regulator of murein genes (VIMSS) compare
b4218 ytfL -0.8 -4.5 predicted inner membrane protein (NCBI) compare
b0685 ybfE -0.8 -1.6 orf, hypothetical protein (VIMSS) compare
b3199 yrbK -0.8 -2.3 hypothetical protein (NCBI) compare
b0406 tgt -0.8 -3.3 queuine tRNA-ribosyltransferase (NCBI) compare
b0999 cbpM -0.8 -2.1 modulator of CbpA co-chaperone (NCBI) compare
b4647 mokA -0.8 -0.5 no description compare
b0225 yafQ -0.8 -2.5 predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI) compare
b3649 rpoZ -0.7 -2.2 DNA-directed RNA polymerase subunit omega (NCBI) compare
b1542 ydfI -0.7 -2.2 predicted mannonate dehydrogenase (NCBI) compare
b4698 mgrR -0.7 -1.7 sRNA antisense regulator down regulates YgdQ and EptB, PhoPQ-regulated, Hfq-dependent; affects sensitivity to antimicrobial peptides (RefSeq) compare
b4294 insA-7 -0.7 -1.7 KpLE2 phage-like element; IS1 repressor protein InsA (NCBI) compare
b2330 yfcB -0.7 -0.7 putative adenine-specific methylase (VIMSS) compare
b2683 ygaH -0.7 -2.0 predicted inner membrane protein (NCBI) compare
b1828 b1828 -0.7 -3.8 putative transport protein (VIMSS) compare
b1358 ydaT -0.7 -0.8 Rac prophage; predicted protein (NCBI) compare
b1362 rzpR -0.7 -1.6 putative Rac prophage endopeptidase (VIMSS) compare
b4493 gapC -0.7 -1.6 no description compare
b0001 thrL -0.7 -1.4 thr operon leader peptide (NCBI) compare
b4180 rlmB -0.7 -3.8 23S rRNA (Gm2251)-methyltransferase (NCBI) compare
b2007 yeeX -0.7 -1.4 conserved protein (RefSeq) compare
b1685 ydiH -0.7 -1.0 predicted protein (RefSeq) compare
b2915 yqfE -0.7 -1.6 hypothetical protein (RefSeq) compare
b3617 kbl -0.7 -4.2 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b1583 ynfB -0.7 -1.8 hypothetical protein (NCBI) compare
b0247 ykfG -0.7 -1.2 CP4-6 prophage; predicted DNA repair protein (NCBI) compare
b1874 cutC -0.7 -1.3 copper homeostasis protein (NCBI) compare
b4000 hupA -0.7 -2.9 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3726 pstA -0.7 -2.5 phosphate transporter subunit (NCBI) compare
b2329 aroC -0.7 -2.6 chorismate synthase (NCBI) compare
b1693 aroD -0.7 -2.7 3-dehydroquinate dehydratase (NCBI) compare
b2682 ygaZ -0.7 -2.1 predicted transporter (NCBI) compare
b3863 polA -0.7 -0.4 DNA polymerase I (NCBI) compare
b1380 ldhA -0.7 -1.7 D-lactate dehydrogenase (NCBI) compare
b4393 trpR -0.7 -3.2 Trp operon repressor (NCBI) compare
b2029 gnd -0.7 -3.6 6-phosphogluconate dehydrogenase (NCBI) compare
b2258 b2258 -0.7 -0.8 putative transport/receptor protein (VIMSS) compare
b3616 tdh -0.7 -3.3 L-threonine 3-dehydrogenase (NCBI) compare
b4603 rseX -0.7 -0.6 sRNA regulating ompA and ompC translation, with Hfq (RefSeq) compare
b1658 purR -0.7 -2.4 DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI) compare
b2792 yqcC -0.7 -2.3 hypothetical protein (NCBI) compare
b4481 rffT -0.7 -2.3 4-alpha-L-fucosyltransferase (NCBI) compare
b3131 agaR -0.7 -2.2 DNA-binding transcriptional dual regulator (NCBI) compare
b3858 yihD -0.7 -1.0 hypothetical protein (NCBI) compare
b3706 trmE -0.7 -1.0 tRNA modification GTPase (NCBI) compare
b1366 ydaY -0.6 -2.0 Rac prophage; predicted protein (RefSeq) compare
b3537 yhjT -0.6 -1.8 orf, hypothetical protein (VIMSS) compare
b0535 fimZ -0.6 -1.0 predicted DNA-binding transcriptional regulator (NCBI) compare
b3611 yibN -0.6 -2.6 predicted rhodanese-related sulfurtransferase (NCBI) compare
b4011 yjaA -0.6 -1.8 hypothetical protein (NCBI) compare
b0339 cynT -0.6 -3.2 carbonic anhydrase (NCBI) compare
b4245 pyrB -0.6 -3.8 aspartate carbamoyltransferase catalytic subunit (NCBI) compare
b1821 b1821 -0.6 -1.1 orf, hypothetical protein (VIMSS) compare
b3613 yibP -0.6 -0.8 putative membrane protein (VIMSS) compare
b0145 dksA -0.6 -1.0 DNA-binding transcriptional regulator of rRNA transcription, DnaK suppressor protein (NCBI) compare
b2284 nuoF -0.6 -2.2 NADH:ubiquinone oxidoreductase, chain F (NCBI) compare
b4006 purH -0.6 -4.1 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b0430 cyoC -0.6 -2.1 cytochrome o ubiquinol oxidase subunit III (NCBI) compare
b3101 yqjF -0.6 -2.0 orf, hypothetical protein (VIMSS) compare
b3916 pfkA -0.6 -0.6 6-phosphofructokinase (NCBI) compare
b1620 malI -0.6 -1.4 DNA-binding transcriptional repressor (NCBI) compare
b2071 yegJ -0.6 -1.0 hypothetical protein (NCBI) compare
b3259 prmA -0.6 -2.3 ribosomal protein L11 methyltransferase (NCBI) compare
b3785 wzzE -0.6 -4.3 putative transport protein (VIMSS) compare


Specific Phenotypes

For 3 genes in this experiment