Experiment set25S3 for Escherichia coli BW25113

Compare to:

1/2ON stiff

200 most important genes:

  gene name fitness t score description  
b0088 murD -5.3 -3.6 UDP-N-acetylmuramoyl-L-alanyl-D-glutamatesynthetase (NCBI) compare
b3961 oxyR -4.0 -16.8 DNA-binding transcriptional dual regulator (NCBI) compare
b3169 nusA -3.3 -3.1 transcription elongation factor NusA (NCBI) compare
b0907 serC -3.3 -8.7 phosphoserine aminotransferase (NCBI) compare
b0912 ihfB -3.2 -5.7 integration host factor subunit beta (NCBI) compare
b0438 clpX -3.1 -4.7 ATP-dependent protease ATP-binding subunit (NCBI) compare
b3035 tolC -2.9 -10.2 outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS) compare
b0437 clpP -2.9 -3.4 ATP-dependent Clp protease proteolytic subunit (NCBI) compare
b1105 ycfM -2.5 -5.3 predicted outer membrane lipoprotein (NCBI) compare
b1865 nudB -2.3 -1.5 dATP pyrophosphohydrolase (NCBI) compare
b1192 ldcA -2.2 -2.9 L,D-carboxypeptidase A (NCBI) compare
b2912 ygfA -2.1 -2.0 putative ligase (VIMSS) compare
b3868 glnG -2.1 -8.7 fused DNA-binding response regulator in two-component regulatory system with GlnL: response regulator/sigma54 interaction protein (NCBI) compare
b3405 ompR -2.1 -3.0 osmolarity response regulator (NCBI) compare
b3396 mrcA -2.0 -17.5 peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS) compare
b0149 mrcB -2.0 -11.3 penicillin-binding protein 1b (NCBI) compare
b4414 tff -2.0 -4.4 unknown RNA (NCBI) compare
b2498 upp -1.9 -2.2 uracil phosphoribosyltransferase (VIMSS) compare
b2017 yefM -1.9 -1.9 orf, hypothetical protein (VIMSS) compare
b2518 ndk -1.9 -2.8 nucleoside diphosphate kinase (NCBI) compare
b3500 gor -1.9 -2.3 glutathione reductase (NCBI) compare
b3790 rffC -1.7 -4.2 TDP-fucosamine acetyltransferase (RefSeq) compare
b0049 apaH -1.7 -2.3 diadenosinetetraphosphatase (NCBI) compare
b4701 sokX -1.6 -1.0 sok-related sRNA, function unknown (RefSeq) compare
b4503 yafF -1.6 -1.0 no description compare
b0386 proC -1.6 -4.1 pyrroline-5-carboxylate reductase (NCBI) compare
b0990 cspG -1.6 -1.0 DNA-binding transcriptional regulator (NCBI) compare
b3164 pnp -1.6 -3.5 polynucleotide phosphorylase/polyadenylase (RefSeq) compare
b3390 aroK -1.5 -1.8 shikimate kinase I (NCBI) compare
b0741 pal -1.5 -2.1 peptidoglycan-associated outer membrane lipoprotein (NCBI) compare
b2330 yfcB -1.5 -1.3 putative adenine-specific methylase (VIMSS) compare
b0740 tolB -1.5 -2.3 translocation protein TolB precursor (NCBI) compare
b3443 yrhA -1.5 -2.4 orf, hypothetical protein (VIMSS) compare
b0439 lon -1.5 -3.7 DNA-binding ATP-dependent protease La (NCBI) compare
b1175 minD -1.4 -4.2 membrane ATPase of the MinC-MinD-MinE system (NCBI) compare
b0683 fur -1.4 -1.4 ferric uptake regulator (NCBI) compare
b3147 yraM -1.4 -11.0 hypothetical protein (NCBI) compare
b1830 prc -1.4 -2.2 carboxy-terminal protease for penicillin-binding protein 3 (NCBI) compare
b0015 dnaJ -1.4 -7.5 chaperone Hsp40, co-chaperone with DnaK (NCBI) compare
b1863 ruvC -1.4 -0.9 Holliday junction resolvase (NCBI) compare
b1085 b1085 -1.3 -2.5 orf, hypothetical protein (VIMSS) compare
b2215 ompC -1.3 -6.4 outer membrane porin protein C (NCBI) compare
b1088 yceD -1.3 -4.3 hypothetical protein (NCBI) compare
b3839 tatC -1.3 -5.5 TatABCE protein translocation system subunit (NCBI) compare
b3781 trxA -1.3 -4.5 thioredoxin 1 (VIMSS) compare
b3636 rpmG -1.2 -2.3 50S ribosomal protein L33 (NCBI) compare
b0767 ybhE -1.2 -3.4 6-phosphogluconolactonase (NCBI) compare
b2528 iscA -1.2 -2.4 iron-sulfur cluster assembly protein (NCBI) compare
b1291 sapD -1.2 -1.1 predicted antimicrobial peptide transporter subunit (NCBI) compare
b3652 recG -1.2 -7.2 ATP-dependent DNA helicase (NCBI) compare
b3764 yifE -1.2 -4.6 hypothetical protein (NCBI) compare
b3791 rffA -1.2 -4.5 TDP-4-oxo-6-deoxy-D-glucose transaminase (NCBI) compare
b1109 ndh -1.2 -3.2 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) compare
b2259 pmrD -1.2 -1.4 polymyxin resistance protein B (VIMSS) compare
b1607 ydgC -1.1 -1.9 conserved inner membrane protein associated with alginate biosynthesis (NCBI) compare
b0730 mngR -1.1 -1.3 DNA-binding transcriptional dual regulator, fatty-acyl-binding (NCBI) compare
b1613 manA -1.1 -1.5 mannose-6-phosphate isomerase (VIMSS) compare
b1286 rnb -1.1 -1.6 exoribonuclease II (NCBI) compare
b3387 dam -1.0 -6.9 DNA adenine methylase (NCBI) compare
b1176 minC -1.0 -1.9 septum formation inhibitor (NCBI) compare
b4480 hdfR -1.0 -4.7 transcriptional regulator HdfR (NCBI) compare
b0862 artQ -1.0 -2.7 arginine transporter subunit (NCBI) compare
b3341 rpsG -1.0 -1.3 30S ribosomal protein S7 (NCBI) compare
b1600 mdtJ -1.0 -1.0 multidrug efflux system transporter (NCBI) compare
b2808 gcvA -1.0 -2.5 DNA-binding transcriptional dual regulator (NCBI) compare
b4203 rplI -1.0 -3.8 50S ribosomal protein L9 (NCBI) compare
b1804 rnd -1.0 -3.3 ribonuclease D (NCBI) compare
b3649 rpoZ -1.0 -2.9 DNA-directed RNA polymerase subunit omega (NCBI) compare
b0014 dnaK -1.0 -1.4 molecular chaperone DnaK (NCBI) compare
b3650 spoT -1.0 -5.6 bifunctional (p)ppGpp synthetase II/ guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase (NCBI) compare
b2947 gshB -1.0 -3.4 glutathione synthetase (NCBI) compare
b4163 glyV -1.0 -1.6 tRNA-Gly (NCBI) compare
b1138 ymfE -0.9 -1.3 e14 prophage; predicted inner membrane protein (NCBI) compare
b0051 ksgA -0.9 -2.6 dimethyladenosine transferase (NCBI) compare
b0222 lpcA -0.9 -1.8 phosphoheptose isomerase (NCBI) compare
b3163 nlpI -0.9 -2.6 hypothetical protein (NCBI) compare
b1566 flxA -0.9 -2.2 Qin prophage; predicted protein (NCBI) compare
b2951 yggS -0.9 -3.8 predicted enzyme (NCBI) compare
b0161 degP -0.9 -4.8 serine endoprotease (protease Do), membrane-associated (NCBI) compare
b1103 ycfF -0.9 -1.2 orf, hypothetical protein (VIMSS) compare
b3616 tdh -0.9 -4.3 L-threonine 3-dehydrogenase (NCBI) compare
b1281 pyrF -0.9 -2.0 orotidine 5'-phosphate decarboxylase (NCBI) compare
b3162 deaD -0.9 -1.8 inducible ATP-independent RNA helicase (VIMSS) compare
b2910 zapA -0.9 -4.1 protein that localizes to the cytokinetic ring (NCBI) compare
b3794 rffM -0.8 -3.6 putative UDP-N-acetyl-D-mannosaminuronic acid transferase (NCBI) compare
b0605 ahpC -0.8 -2.1 alkyl hydroperoxide reductase, C22 subunit (NCBI) compare
b1850 eda -0.8 -1.2 keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase (NCBI) compare
b2183 rsuA -0.8 -2.7 16S rRNA pseudouridylate 516 synthase (NCBI) compare
b2318 truA -0.8 -1.4 tRNA pseudouridine synthase A (NCBI) compare
b1984 asnW -0.8 -1.0 tRNA-Asn (NCBI) compare
b4481 rffT -0.8 -2.6 4-alpha-L-fucosyltransferase (NCBI) compare
b3871 bipA -0.8 -6.0 GTP-binding protein (NCBI) compare
b3933 ftsN -0.8 -4.6 essential cell division protein (NCBI) compare
b2683 ygaH -0.8 -2.4 predicted inner membrane protein (NCBI) compare
b3183 obgE -0.8 -0.9 GTPase involved in cell partioning and DNA repair (NCBI) compare
b0627 tatE -0.8 -1.8 twin arginine translocase protein E (NCBI) compare
b1084 rne -0.8 -2.6 fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI) compare
b2181 yejG -0.8 -2.0 hypothetical protein (NCBI) compare
b2093 gatB -0.8 -2.1 galactitol-specific enzyme IIB component of PTS (NCBI) compare
b2189 proL -0.8 -1.3 tRNA-Pro (NCBI) compare
b4000 hupA -0.8 -3.1 HU, DNA-binding transcriptional regulator, alpha subunit (NCBI) compare
b3778 rep -0.8 -3.4 DNA helicase and single-stranded DNA-dependent ATPase (NCBI) compare
b1362 rzpR -0.8 -1.7 putative Rac prophage endopeptidase (VIMSS) compare
b1709 btuD -0.8 -1.0 vitamin B12-transporter ATPase (NCBI) compare
b0828 iaaA -0.8 -3.7 L-asparaginase (NCBI) compare
b4552 yrhC -0.8 -0.7 no description compare
b2356 yfdM -0.8 -1.8 orf, hypothetical protein (VIMSS) compare
b3786 rffE -0.8 -4.8 UDP-N-acetyl glucosamine-2-epimerase (NCBI) compare
b2281 nuoI -0.7 -2.0 NADH dehydrogenase subunit I (NCBI) compare
b3199 yrbK -0.7 -2.3 hypothetical protein (NCBI) compare
b3784 rfe -0.7 -4.8 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase (NCBI) compare
b4006 purH -0.7 -5.2 bifunctionalphosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (NCBI) compare
b0920 ycbC -0.7 -2.7 conserved inner membrane protein (NCBI) compare
b3026 qseC -0.7 -1.0 sensory histidine kinase in two-component regulatory system with QseB (NCBI) compare
b2479 gcvR -0.7 -3.0 transcriptional regulation of gcv operon (VIMSS) compare
b3613 yibP -0.7 -0.7 putative membrane protein (VIMSS) compare
b2895 fldB -0.7 -1.8 flavodoxin 2 (NCBI) compare
b1907 tyrP -0.7 -2.7 tyrosine transporter (NCBI) compare
b2444 yffM -0.7 -1.3 CPZ-55 prophage; predicted protein (NCBI) compare
b2744 surE -0.7 -3.7 acid phosphatase (NCBI) compare
b1524 yneH -0.7 -0.7 predicted glutaminase (NCBI) compare
b1727 yniC -0.7 -1.5 predicted hydrolase (NCBI) compare
b1882 cheY -0.7 -0.3 chemotaxis regulator transmitting signal to flagellar motor component (NCBI) compare
b1971 yedY -0.7 -2.7 hypothetical protein (NCBI) compare
b1418 cybB -0.7 -2.0 cytochrome b(561) (VIMSS) compare
b3863 polA -0.7 -0.4 DNA polymerase I (NCBI) compare
b4633 xisD -0.7 -2.8 no description compare
b0059 hepA -0.7 -5.9 ATP-dependent helicase HepA (NCBI) compare
b4218 ytfL -0.7 -4.5 predicted inner membrane protein (NCBI) compare
b2829 ptsP -0.7 -5.1 fused PTS enzyme: PEP-protein phosphotransferase (enzyme I)/GAF domain containing protein (NCBI) compare
b2024 hisA -0.7 -1.5 N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS) compare
b3870 glnA -0.7 -1.8 glutamine synthetase (NCBI) compare
b3149 yraO -0.7 -3.0 DnaA initiator-associating factor for replication initiation (NCBI) compare
b0339 cynT -0.7 -2.7 carbonic anhydrase (NCBI) compare
b0888 trxB -0.7 -1.2 thioredoxin reductase, FAD/NAD(P)-binding (NCBI) compare
b1742 b1742 -0.7 -1.8 orf, hypothetical protein (VIMSS) compare
b3260 dusB -0.7 -3.5 tRNA-dihydrouridine synthase B (NCBI) compare
b4529 ydbJ -0.7 -0.7 hypothetical protein (NCBI) compare
b2083 yegZ -0.7 -1.7 orf, hypothetical protein (VIMSS) compare
b4201 priB -0.7 -1.4 primosomal replication protein N (NCBI) compare
b0019 nhaA -0.7 -4.3 pH-dependent sodium/proton antiporter (NCBI) compare
b3157 yhbT -0.7 -2.9 predicted lipid carrier protein (NCBI) compare
b3816 corA -0.7 -4.4 magnesium/nickel/cobalt transporter (NCBI) compare
b4178 yjeB -0.7 -2.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b3787 rffD -0.7 -3.9 UDP-N-acetyl-D-mannosaminuronic acid dehydrogenase (NCBI) compare
b1693 aroD -0.7 -2.6 3-dehydroquinate dehydratase (NCBI) compare
b2501 ppk -0.7 -3.1 polyphosphate kinase (NCBI) compare
b3916 pfkA -0.6 -0.8 6-phosphofructokinase (NCBI) compare
b2689 yqaA -0.6 -1.9 conserved inner membrane protein (NCBI) compare
b4457 csrC -0.6 -2.5 regulatory RNA (NCBI) compare
b2784 relA -0.6 -4.2 (p)ppGpp synthetase I/GTP pyrophosphokinase (NCBI) compare
b2915 yqfE -0.6 -1.9 hypothetical protein (RefSeq) compare
b1686 ydiI -0.6 -1.3 hypothetical protein (NCBI) compare
b1504 ydeS -0.6 -2.3 predicted fimbrial-like adhesin protein (NCBI) compare
b1480 sra -0.6 -0.8 30S ribosomal subunit protein S22 (NCBI) compare
b3545 proK -0.6 -1.0 tRNA-Pro (NCBI) compare
b0405 queA -0.6 -3.4 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI) compare
b3438 gntR -0.6 -3.8 DNA-binding transcriptional repressor (NCBI) compare
b0881 clpS -0.6 -1.0 ATP-dependent Clp protease adaptor protein ClpS (NCBI) compare
b1095 fabF -0.6 -3.7 3-oxoacyl-(acyl carrier protein) synthase (NCBI) compare
b1522 yneF -0.6 -2.8 predicted diguanylate cyclase (NCBI) compare
b4369 leuP -0.6 -2.0 tRNA-Leu (NCBI) compare
b2435 amiA -0.6 -2.9 N-acetylmuramoyl-l-alanine amidase I (NCBI) compare
b2512 yfgL -0.6 -2.5 protein assembly complex, lipoprotein component (NCBI) compare
b3617 kbl -0.6 -3.8 2-amino-3-ketobutyrate coenzyme A ligase (NCBI) compare
b1942 fliJ -0.6 -1.0 flagellar biosynthesis chaperone (NCBI) compare
b2576 srmB -0.6 -1.1 ATP-dependent RNA helicase (NCBI) compare
b4406 yaeP -0.6 -1.1 hypothetical protein (NCBI) compare
b0710 ybgI -0.6 -2.4 conserved metal-binding protein (NCBI) compare
b0606 ahpF -0.6 -2.9 alkyl hydroperoxide reductase, F52a subunit; detoxification of hydroperoxides (VIMSS) compare
b3806 cyaA -0.6 -2.7 adenylate cyclase (NCBI) compare
b1819 manZ -0.6 -2.4 mannose-specific enzyme IID component of PTS (NCBI) compare
b3726 pstA -0.6 -2.4 phosphate transporter subunit (NCBI) compare
b0082 mraW -0.6 -1.9 S-adenosyl-methyltransferase (NCBI) compare
b0895 dmsB -0.6 -2.6 dimethyl sulfoxide reductase, anaerobic, subunit B (NCBI) compare
b0942 ycbU -0.6 -2.1 predicted fimbrial-like adhesin protein (NCBI) compare
b1335 ogt -0.6 -1.7 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) compare
b1104 ycfL -0.6 -0.8 hypothetical protein (NCBI) compare
b4450 arcZ -0.6 -2.1 sRNA antisense regulator of tpx and sdaA, Hfq-dependent (RefSeq) compare
b3100 yqjK -0.6 -2.2 hypothetical protein (NCBI) compare
b1332 ynaJ -0.6 -0.3 predicted inner membrane protein (NCBI) compare
b0407 yajC -0.6 -1.3 preprotein translocase subunit YajC (NCBI) compare
b1827 kdgR -0.6 -1.7 predicted DNA-binding transcriptional regulator (NCBI) compare
b0219 yafV -0.6 -2.4 predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI) compare
b2209 eco -0.6 -1.0 ecotin precursor (NCBI) compare
b1874 cutC -0.6 -1.1 copper homeostasis protein (NCBI) compare
b1171 b1171 -0.6 -1.0 orf, hypothetical protein (VIMSS) compare
b0435 bolA -0.6 -2.9 possible regulator of murein genes (VIMSS) compare
b4202 rpsR -0.6 -0.6 30S ribosomal protein S18 (NCBI) compare
b3860 dsbA -0.6 -4.3 periplasmic protein disulfide isomerase I (NCBI) compare
b2236 yfaE -0.6 -1.7 predicted 2Fe-2S cluster-containing protein (NCBI) compare
b4053 alr -0.6 -4.0 alanine racemase (NCBI) compare
b3414 gntY -0.6 -2.7 predicted gluconate transport associated protein (NCBI) compare
b1324 tpx -0.6 -2.0 thiol peroxidase (NCBI) compare
b2906 visC -0.6 -2.9 hypothetical protein (NCBI) compare
b1676 pykF -0.6 -1.6 pyruvate kinase (NCBI) compare
b4653 yicT -0.6 -1.4 no description compare
b3446 yrhB -0.6 -2.4 hypothetical protein (NCBI) compare
b3706 trmE -0.6 -1.2 tRNA modification GTPase (NCBI) compare
b3611 yibN -0.5 -2.4 predicted rhodanese-related sulfurtransferase (NCBI) compare


Specific Phenotypes

For 2 genes in this experiment