Experiment set25IT057 for Pseudomonas simiae WCS417
P. simiae Grant Run MOI 10
Group: phageMedia: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 1/18/23
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Potassium homeostasis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
- Geraniol degradation
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Brassinosteroid biosynthesis
- Caprolactam degradation
- Biosynthesis of terpenoids and steroids
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| adipate degradation | 5 | 5 | 1 |
| fatty acid salvage | 6 | 6 | 1 |
| oleate β-oxidation | 35 | 30 | 3 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 1 |