Experiment set25IT047 for Pseudomonas putida KT2440

200 most detrimental genes:

gene	name	fitness	t score	description
PP_1655		+3.1	6.6	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD
PP_1656		+3.0	8.7	ATP:GTP 3'-pyrophosphotransferase
PP_3241		+2.2	3.5	conserved exported protein of unknown function
PP_4268		+1.8	1.5	nucleoid-associated broad specificity regulator
PP_4797		+1.7	2.4	conserved protein of unknown function
PP_2403		+1.6	2.7	Chemotaxis protein CheY
PP_5054		+1.6	2.5	glutaredoxin 3
PP_4136		+1.5	0.9	Transcriptional regulator, LuxR family
PP_4701		+1.5	1.8	glucose-6-phosphate isomerase
PP_3098		+1.5	2.0	conserved protein of unknown function
PP_2060		+1.5	2.1	DNA-binding transcriptional dual regulator - SoxR-reversible one-electron oxidation of its [2Fe-2S] cluster, NADPH-dependent
PP_1269		+1.4	1.9	Universal stress protein family
PP_1381		+1.4	1.9	4-carboxymuconolactone decarboxylase
PP_3938		+1.4	3.2	conserved protein of unknown function
PP_0377		+1.4	2.2	coenzyme PQQ synthesis protein D
PP_5114		+1.4	1.7	putative adenine methyltransferase
PP_3898		+1.3	2.8	conserved protein of unknown function
PP_0640		+1.3	1.8	conserved protein of unknown function
PP_1876		+1.3	2.2	putative ATP/GTP-binding protein of the AAA+ family
PP_3419		+1.3	4.6	Sigma-54 dependent transcriptional regulator/response regulator
PP_2740		+1.2	2.4	Transcriptional regulator, MerR family
PP_0775		+1.2	2.0	conserved protein of unknown function
PP_5017		+1.2	1.5	Sec-independent protein translocase protein TatB
PP_3971		+1.2	0.9	conserved protein of unknown function
PP_1647		+1.2	4.6	conserved protein of unknown function
PP_3392		+1.1	2.3	conserved protein of unknown function
PP_4681		+1.1	1.7	conserved protein of unknown function
PP_4373		+1.1	7.7	Transcriptional regulator FleQ
PP_5748		+1.1	1.0	cytochrome c heme lyase subunit
PP_2999		+1.1	1.6	Glyoxalase family protein
PP_3892		+1.1	1.4	conserved protein of unknown function
PP_3840		+1.1	1.7	conserved protein of unknown function
PP_4808		+1.1	2.1	23S rRNA m3psi1915 methyltransferase
PP_5571		+1.1	1.4	conserved protein of unknown function
PP_0787		+1.1	2.4	quinolinate phosphoribosyltransferase (decarboxylating) monomer
PP_1135		+1.1	4.3	conserved exported protein of unknown function
PP_4848		+1.0	2.8	Curved DNA-binding protein
PP_5168		+1.0	2.8	sulfate/thiosulfate import ATP-binding protein
PP_2747		+1.0	2.2	conserved protein of unknown function
PP_4233		+1.0	2.7	putative Oxidoreductase, small subunit
PP_5661		+1.0	2.2	putative lipoprotein
PP_3523		+1.0	2.2	conserved protein of unknown function
PP_2970		+1.0	1.9	conserved exported protein of unknown function
PP_0291		+1.0	2.8	conserved protein of unknown function
PP_1255		+1.0	2.8	putative cis-4-hydroxy-D-proline oxidase
PP_4074		+1.0	3.9	conserved protein of unknown function
PP_0999		+1.0	2.2	carbamate kinase
PP_0566		+1.0	1.0	translation-related factor
PP_2429		+1.0	1.9	putative Membrane protein
PP_4098		+0.9	0.9	UvrABC system protein C
PP_5453		+0.9	0.6	conserved protein of unknown function
PP_0653		+0.9	1.2	conserved protein of unknown function
PP_1404		+0.9	0.9	conserved protein of unknown function
PP_4223		+0.9	0.9	diaminobutyrate-2-oxoglutarate transaminase
PP_3398		+0.9	1.6	putative Curli fiber surface-exposed nucleator CsgB
PP_3477		+0.9	2.9	Putative secretion type II protein related to protein G
PP_4227		+0.9	1.5	conserved protein of unknown function
PP_5027		+0.9	1.2	D-tyrosyl-tRNA(Tyr) deacylase
PP_0108		+0.9	1.8	conserved protein of unknown function
PP_0611		+0.9	2.6	Type IV pili biogenesis protein PilE
PP_3757		+0.9	1.2	Chemotaxis protein CheY
PP_3810		+0.9	1.9	conserved protein of unknown function
PP_3110		+0.9	1.0	protein of unknown function
PP_4581		+0.9	2.8	putative amidotransferase
PP_5139		+0.9	2.6	cadmium translocating P-type ATPase
PP_2575		+0.9	1.6	conserved exported protein of unknown function
PP_0632		+0.8	2.0	leader peptidase/N-methyltransferase
PP_3307		+0.8	1.2	conserved protein of unknown function
PP_1706		+0.8	1.8	Nitrite reductase
PP_3248		+0.8	1.6	Dyp-type peroxidase family protein
PP_5537		+0.8	1.4	conserved membrane protein of unknown function
PP_5475		+0.8	0.5	conserved protein of unknown function
PP_2442		+0.8	1.8	conserved protein of unknown function, DUF480 family
PP_1293		+0.8	0.8	molybdopterin synthase small subunit
PP_1813		+0.8	1.4	ComEA-related protein
PP_2070		+0.8	1.5	Transcriptional regulator, AraC family
PP_0152		+0.8	0.9	conserved exported protein of unknown function
PP_2197		+0.8	2.1	conserved exported protein of unknown function
PP_3444		+0.8	3.3	Glyoxalase family protein
PP_3654		+0.8	2.6	leucine-responsive regulatory protein
PP_2539		+0.8	2.4	Transcriptional regulator, LysR family
PP_4254		+0.8	0.5	conserved protein of unknown function
PP_3421		+0.8	4.0	Sensor histidine kinase
PP_5121		+0.8	2.3	conserved exported protein of unknown function
PP_0839		+0.8	1.2	tRNA Cm32/Um32 methyltransferase
PP_0989		+0.8	1.4	glycine cleavage system H protein 1
PP_4833		+0.7	2.2	SURF1 domain protein
PP_5712		+0.7	1.6	conserved protein of unknown function
PP_1096		+0.7	1.8	putative electron transport complex protein
PP_0025		+0.7	0.9	hypothetical protein
PP_0627		+0.7	1.7	conserved protein of unknown function
PP_5508		+0.7	0.9	conserved protein of unknown function
PP_0556		+0.7	1.1	Acetoin catabolism protein
PP_3233		+0.7	1.7	transcriptional regulator with 4Fe-4S cluster, Crp/Fnr family
PP_2580		+0.7	1.5	conserved protein of unknown function
PP_2025		+0.7	1.2	Exonuclease SbcD
PP_0610		+0.7	2.9	conserved exported protein of unknown function
PP_3121		+0.7	2.5	transcriptional regulator, LysR family
PP_3320		+0.7	1.3	conserved protein of unknown function
PP_0626		+0.7	3.1	NADH dehydrogenase
PP_0979		+0.7	1.2	DNA polymerase III subunit chi
PP_4348		+0.7	2.0	putative Cystathionine beta-lyase
PP_4660		+0.7	1.2	Transcriptional regulator, LysR family
PP_0086		+0.7	1.2	conserved protein of unknown function
PP_0684		+0.7	2.9	FKBP-type peptidyl-prolyl cis-trans isomerase
PP_2677		+0.7	1.7	conserved exported protein of unknown function
PP_1478		+0.7	3.5	putative Xenobiotic reductase
PP_0202		+0.7	1.5	CBS domain-containing protein
PP_5205		+0.7	2.8	conserved protein of unknown function
PP_2226		+0.7	1.4	putative K(+)/H(+) antiporter subunit F
PP_3064		+0.7	1.8	Phage tail protein
PP_3055		+0.7	1.9	Pyocin R2_PP, tail formation protein
PP_3204		+0.7	2.3	conserved protein of unknown function
PP_3423		+0.7	3.0	Type II secretion pathway protein XcpT
PP_3672		+0.7	1.3	conserved protein of unknown function
PP_4513		+0.7	1.8	conserved protein of unknown function
PP_3643		+0.7	1.1	putative Oxidoreductase
PP_4406		+0.7	0.7	conserved protein of unknown function
PP_2764		+0.7	0.9	FMN reductase (NADPH)
PP_0886		+0.7	1.1	conserved exported protein of unknown function
PP_5610		+0.7	1.1	protein of unknown function
PP_3396		+0.7	3.7	conserved exported protein of unknown function
PP_1745		+0.7	2.1	Oxidoreductase, short chain dehydrogenase/reductase family
PP_2749		+0.7	1.3	putative Branched-chain amino acid ABC transporter, permease protein
PP_5582		+0.7	0.9	conserved protein of unknown function
PP_2616		+0.7	1.2	conserved protein of unknown function
PP_3397		+0.7	4.3	conserved exported protein of unknown function
PP_4609		+0.7	2.6	conserved membrane protein of unknown function
PP_4569		+0.7	1.7	conserved protein of unknown function
PP_0151		+0.7	1.0	conserved exported protein of unknown function
PP_1947		+0.7	2.3	conserved protein of unknown function
PP_4196		+0.7	1.5	conserved exported protein of unknown function
PP_4423		+0.7	2.2	conserved protein of unknown function
PP_0087		+0.7	1.5	conserved exported protein of unknown function
PP_2455		+0.7	1.9	ribose ABC transporter - ATP-binding subunit
PP_2387		+0.7	0.9	conserved protein of unknown function
PP_0274		+0.7	0.4	putative transcriptional regulator containing HTH domain
PP_1454		+0.7	1.5	conserved protein of unknown function
PP_1744		+0.7	2.1	conserved exported protein of unknown function
PP_0194		+0.6	1.4	Transcriptional regulatory protein AlgP
PP_2132		+0.6	1.3	Universal stress protein
PP_0195		+0.6	1.3	conserved protein of unknown function
PP_5014		+0.6	1.0	Phosphoribosyl-AMP cyclohydrolase
PP_3399		+0.6	3.7	minor curlin subunit CsgB, nucleation component of curlin monomers
PP_1568		+0.6	0.7	conserved protein of unknown function
PP_1344		+0.6	0.8	conserved protein of unknown function
PP_2737		+0.6	1.1	Oxidoreductase, short-chain dehydrogenase/reductase family
PP_5505		+0.6	1.0	conserved transmembrane protein of unknown function
PP_0945		+0.6	0.9	conserved protein of unknown function
PP_5293		+0.6	1.9	conserved protein of unknown function
PP_1855		+0.6	1.0	conserved exported protein of unknown function
PP_3472		+0.6	2.7	curli secretion and assembly complex lipoprotein / curli secretion channel
PP_0090		+0.6	1.7	conserved exported protein of unknown function
PP_4519		+0.6	3.8	Agglutination protein
PP_1790		+0.6	1.5	putative Acylneuraminate cytidylyltransferase
PP_2227		+0.6	1.4	putative Multicomponent potassium-proton antiporter, subunit E
PP_1630		+0.6	1.6	Regulatory protein RecX
PP_5381		+0.6	1.2	hypothetical protein
PP_5628		+0.6	1.0	conserved protein of unknown function
PP_3574		+0.6	1.6	conserved protein of unknown function
PP_2592		+0.6	0.9	Ferric siderophore ABC transporter, ATP-binding protein
PP_2422		+0.6	2.0	conserved protein of unknown function
PP_1552		+0.6	1.3	putative Phage replication protein
PP_1892		+0.6	0.9	conserved protein of unknown function
PP_5471		+0.6	0.7	putative response regulator receiver protein
PP_1650		+0.6	4.9	Sensor protein GacS
PP_0593		+0.6	0.5	putative ribosome-binding factor
PP_3449		+0.6	1.0	conserved exported protein of unknown function
PP_1544		+0.6	1.4	conserved protein of unknown function
PP_0167		+0.6	4.4	Toxin secretion ATP-binding protein
PP_2083		+0.6	1.3	putative Lipase
PP_3824		+0.6	1.0	conserved exported protein of unknown function
PP_2889		+0.6	2.4	putative Transmembrane regulator PrtR
PP_1833		+0.6	1.9	conserved protein of unknown function
PP_1113		+0.6	2.7	Pyridoxal-phosphate dependent enzyme family protein
PP_2311		+0.6	1.2	Mg-dependent cytoplasmic DNase / RNase
PP_2532		+0.6	2.7	conserved protein of unknown function
PP_1999		+0.6	0.6	membrane protein required for colicin V production
PP_2559		+0.6	2.0	Metalloprotease secretion protein
PP_3684		+0.6	2.2	conserved protein of unknown function
PP_4246		+0.6	2.6	Periplasmic binding protein
PP_5429		+0.6	2.2	protein of unknown function
PP_2107		+0.6	0.7	conserved protein of unknown function
PP_5640		+0.6	1.4	conserved protein of unknown function
PP_0326		+0.6	1.2	sarcosine oxidase subunit gamma
PP_0283		+0.6	2.0	Arginine/ornithine transport ATP-binding protein AotP
PP_0168		+0.6	4.7	putative surface adhesion protein
PP_3384		+0.6	2.1	gluconate 2-dehydrogenase gamma subunit
PP_5426		+0.6	2.0	conserved protein of unknown function
PP_3524		+0.6	1.9	conserved protein of unknown function
PP_4905		+0.6	1.6	Flagellar motor rotation protein
PP_3871		+0.6	1.6	conserved protein of unknown function
PP_4559		+0.6	1.4	peptide deformylase
PP_0991		+0.6	1.8	conserved protein of unknown function
PP_0235		+0.6	1.3	Peroxidase
PP_4502		+0.6	1.2	putative enzyme
PP_0840		+0.6	1.7	serine acetyltransferase
PP_2067		+0.6	1.1	putative EmrB/QacA family drug resistance transporter
PP_4968		+0.6	1.8	DNA ligase B
PP_5546		+0.6	0.8	conserved protein of unknown function

Specific Phenotypes

For 1 genes in this experiment

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs in Pseudomonas putida KT2440

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=288_hrs across organisms