Experiment set25IT022 for Pseudomonas simiae WCS417
P. simiae OR1 MOI 0.1
Group: phageMedia: LB_plus_SM_buffer + Kan (50 ug/ml)
Culturing: fluoroDangl_ML3, 48 well microplate; Epoch2, Aerobic, at 30 (C), shaken=double orbital, continuous, 205cpm
By: Vivek on 8/27/22
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 5 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| N-linked Glycosylation in Bacteria | 1 |
| Protein degradation | 1 |
| Rhamnose containing glycans | 1 |
| Teichoic and lipoteichoic acids biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fatty acid biosynthesis
- Tyrosine metabolism
- Benzoate degradation via hydroxylation
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Nucleotide sugars metabolism
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Porphyrin and chlorophyll metabolism
- Limonene and pinene degradation
- Biosynthesis of unsaturated fatty acids
- Biosynthesis of type II polyketide backbone
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| Arg/N-end rule pathway (eukaryotic) | 14 | 8 | 4 |
| UDP-N-acetyl-β-L-fucosamine biosynthesis | 4 | 2 | 1 |
| UDP-N-acetyl-β-L-quinovosamine biosynthesis | 4 | 1 | 1 |
| CMP-pseudaminate biosynthesis | 6 | 1 | 1 |
| CMP-diacetamido-8-epilegionaminic acid biosynthesis | 7 | 1 | 1 |
| superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis | 24 | 9 | 1 |