Experiment set24S529 for Agrobacterium fabrum C58

LB with Hygromycin B 25 ug/mL

Group: stress
Media: LB + Hygromycin B (25 ug/mL)
Culturing: Agro_ML11b, 24_well_plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 4/26/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 12 genes in this experiment

For stress Hygromycin B in Agrobacterium fabrum C58

For stress Hygromycin B across organisms

SEED Subsystems

Subsystem	#Specific
Bacterial RNA-metabolizing Zn-dependent hydrolases	1
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Glutathione-regulated potassium-efflux system and associated functions	1
Multidrug Resistance Efflux Pumps	1
Peptidoglycan Biosynthesis	1
Potassium homeostasis	1
RNA processing and degradation, bacterial	1
Terminal cytochrome d ubiquinol oxidases	1
Terminal cytochrome oxidases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
tRNA processing	10	10	2