Experiment set24S522 for Agrobacterium fabrum C58

LB with Erythromycin 6.25 ug/mL

Group: stress
Media: LB + Erythromycin (6.25 ug/mL)
Culturing: Agro_ML11b, 24_well_plate, Aerobic, at 28 (C), shaken=200 rpm
By: Mitchell Thompson on 4/26/2025
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 21 genes in this experiment

For stress Erythromycin in Agrobacterium fabrum C58

For stress Erythromycin across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	3
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	2
Conserved gene cluster associated with Met-tRNA formyltransferase	1
Heat shock dnaK gene cluster extended	1
Hfl operon	1
Peptidoglycan Biosynthesis	1
Phosphate metabolism	1
RNA processing and degradation, bacterial	1
Synechocystis experimental	1
Triacylglycerol metabolism	1
Universal GTPases	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Ubiquinone and menaquinone biosynthesis
• Ascorbate and aldarate metabolism
• Histidine metabolism
• Tyrosine metabolism
• Tryptophan metabolism
• Benzoxazinone biosynthesis
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Naphthalene and anthracene degradation
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
phospholipid remodeling (phosphatidylethanolamine, yeast)	4	2	1
tRNA processing	10	10	2