Experiment set24IT035 for Pseudomonas putida KT2440

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Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs

200 most important genes:

  gene name fitness t score description  
PP_0164 -5.3 -8.1 conserved protein of unknown function compare
PP_0293 -3.2 -2.3 Imidazole glycerol phosphate synthase subunit HisF compare
PP_1734 -2.9 -6.2 Probable septum site-determining protein MinC compare
PP_5185 -2.4 -5.0 acetylglutamate kinase-like protein (regulatory domain)/amino acid N-acetyltransferase compare
PP_5289 -2.4 -4.0 Acetylglutamate kinase compare
PP_2378 -2.3 -2.7 Fe/S biogenesis protein NfuA compare
PP_3903 -2.3 -3.4 conserved protein of unknown function compare
PP_4994 -2.1 -5.4 conserved protein of unknown function compare
PP_1733 -2.1 -3.0 membrane ATPase of the MinC-MinD-MinE system compare
PP_5095 -2.1 -1.4 pyrroline-5-carboxylate reductase compare
PP_0888 -2.0 -2.6 Photosynthetic apparatus regulatory protein RegA compare
PP_4120 -1.9 -3.1 NADH-quinone oxidoreductase subunit B compare
PP_4262 -1.9 -2.4 Cytochrome oxidase maturation protein, cbb3-type compare
PP_4700 -1.8 -1.3 pantothenate synthetase compare
PP_5216 -1.8 -2.1 exopolyphosphatase compare
PP_3946 -1.8 -1.9 HTH-type transcriptional repressor NicR compare
PP_1763 -1.7 -3.5 putative oxidoreductase compare
PP_4122 -1.7 -2.1 proton-pumping NADH:ubiquinone oxidoreductase, respiratory complex I, subunit E compare
PP_4726 -1.7 -3.3 Chaperone protein DnaJ compare
PP_5350 -1.6 -3.4 Transcriptional regulator, RpiR family compare
PP_2088 -1.6 -2.0 RNA polymerase sigma factor SigX compare
PP_4131 -1.6 -4.4 NADH-quinone oxidoreductase subunit N compare
PP_4121 -1.6 -3.4 NADH-quinone oxidoreductase subunit C/D compare
PP_0218 -1.6 -7.1 Sensory box protein compare
PP_4379 -1.6 -3.4 Beta-ketoacyl-acyl-carrier-protein synthase I compare
PP_2883 -1.6 -2.1 conserved protein of unknown function compare
PP_0232 -1.6 -1.7 ATP-binding taurine transporter subunit compare
PP_4471 -1.6 -3.7 Magnesium transporter compare
PP_0184 -1.6 -4.3 Argininosuccinate lyase compare
PP_4902 -1.5 -2.9 Oligoribonuclease compare
PP_4993 -1.5 -4.3 Glutathione synthetase compare
PP_2903 -1.5 -2.5 peptidyl-prolyl cis-trans isomerase B (rotamase B) compare
PP_4126 -1.5 -1.4 NADH-quinone oxidoreductase subunit I compare
PP_1088 -1.5 -7.2 Argininosuccinate synthase compare
PP_5015 -1.4 -2.8 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
PP_0001 -1.4 -1.2 probable chromosome-partitioning protein compare
PP_4124 -1.4 -3.5 NADH-quinone oxidoreductase subunit G compare
PP_1439 -1.4 -1.9 conserved exported protein of unknown function compare
PP_5001 -1.4 -2.9 protease HslVU, ATPase component compare
PP_1493 -1.4 -2.4 chemotaxis response regulator protein-glutamate methylesterase of group 3 operon compare
PP_4917 -1.4 -3.7 3',5'-cyclic-nucleotide phosphodiesterase compare
PP_4370 -1.4 -3.7 Flagellar hook-basal body complex protein FliE compare
PP_4004 -1.4 -3.7 DNA translocase FtsK compare
PP_2336 -1.3 -2.2 aconitate hydratase 1 compare
PP_5328 -1.3 -2.0 putative phosphate transport system permease protein compare
PP_4375 -1.3 -7.0 flagellar chaperone, inhibits premature FliC assembly compare
PP_3924 -1.3 -2.5 conserved protein of unknown function compare
PP_5309 -1.3 -4.2 oxidative and nitrosative stress transcriptional dual regulator compare
PP_4720 -1.3 -2.7 putative RNA binding protein compare
PP_0846 -1.3 -0.8 DnaK-like molecular chaperone specific for IscU compare
PP_2302 -1.3 -4.5 DNA-binding, ATP-dependent protease compare
PP_1268 -1.3 -3.3 putative HtrA suppressor protein sohA compare
PP_2433 -1.3 -2.5 conserved protein of unknown function compare
PP_1198 -1.3 -1.9 transcriptional regulator, Cro/CI family (higA antitoxin like) compare
PP_1912 -1.3 -4.2 Phosphate acyltransferase compare
PP_4895 -1.3 -1.9 tRNA dimethylallyltransferase compare
PP_0290 -1.2 -4.1 Imidazole glycerol phosphate synthase subunit HisH compare
PP_4129 -1.2 -2.6 NADH:ubiquinone oxidoreductase, membrane subunit L compare
PP_4353 -1.2 -6.3 Flagellar biosynthetic protein fliR compare
PP_5583 -1.2 -1.5 conserved protein of unknown function compare
PP_2402 -1.2 -1.7 putative Sensor histidine kinase compare
PP_5327 -1.2 -2.7 putative phosphate ABC transporter, permease subunit compare
PP_2341 -1.2 -2.6 6-carboxy-5,6,7,8-tetrahydropterin synthase compare
PP_1208 -1.2 -1.4 conserved protein of unknown function, SlyX family compare
PP_4389 -1.2 -5.5 Flagellar basal-body rod modification protein FlgD compare
PP_1293 -1.1 -1.8 molybdopterin synthase small subunit compare
PP_4941 -1.1 -4.8 conserved exported protein of unknown function compare
PP_4332 -1.1 -2.6 Chemotaxis protein CheW compare
PP_3999 -1.1 -5.0 Uroporphyrinogen-III C-methyltransferase/Precorrin-2 dehydrogenase/Sirohydrochlorin ferrochelatase compare
PP_4338 -1.1 -5.5 Chemotaxis histidine kinase CheA compare
PP_4058 -1.1 -5.6 1,4-alpha-glucan branching enzyme compare
PP_1994 -1.1 -1.9 tRNA pseudouridine (38-40)synthase compare
PP_0065 -1.1 -2.1 NAD-binding component of TrK potassium transporter compare
PP_4874 -1.1 -1.0 50S ribosomal protein L9 compare
PP_4366 -1.1 -7.6 flagellum-specific ATP synthase compare
PP_4964 -1.1 -2.3 D-erythrose 4-phosphate dehydrogenase compare
PP_1559 -1.1 -4.0 Holin compare
PP_4396 -1.1 -2.8 conserved protein of unknown function compare
PP_5198 -1.1 -2.1 conserved protein of unknown function compare
PP_1362 -1.1 -2.7 pyruvate kinase II compare
PP_5329 -1.1 -1.0 putative phosphate ABC transporter, periplasmic phosphate-binding protein compare
PP_4383 -1.1 -3.3 Flagellar P-ring protein compare
PP_3773 -1.1 -2.8 conserved protein of unknown function compare
PP_3409 -1.0 -2.7 putative Cobalamin biosynthesis protein cobE compare
PP_0801 -1.0 -1.3 conserved exported protein of unknown function compare
PP_4365 -1.0 -4.1 Flagellar protein FliJ compare
PP_1410 -1.0 -1.3 conserved protein of unknown function compare
PP_4344 -1.0 -6.2 flagellar export pore protein compare
PP_2414 -1.0 -1.6 conserved exported protein of unknown function compare
PP_2878 -1.0 -1.6 conserved protein of unknown function compare
PP_0063 -1.0 -3.0 putative Lipid A biosynthesis lauroyl acyltransferase compare
PP_4136 -1.0 -1.3 Transcriptional regulator, LuxR family compare
PP_5176 -1.0 -2.1 conserved protein of unknown function compare
PP_1328 -1.0 -1.9 Protein MraZ compare
PP_4358 -1.0 -3.4 flagellar synthesis, switching and energizing component compare
PP_4824 -1.0 -4.2 Sensor histidine kinase/response regulator compare
PP_1215 -1.0 -1.7 Crossover junction endodeoxyribonuclease RuvC compare
PP_4356 -1.0 -2.0 Flagellar protein FliO compare
PP_1598 -1.0 -2.6 regulatory intramembrane protein RIP zinc protease compare
PP_5083 -1.0 -1.9 putative Type IV pili biogenesis protein PilM compare
PP_4376 -1.0 -6.2 flagellar filament capping protein compare
PP_4008 -1.0 -4.5 ATP-dependent serine protease compare
PP_4368 -0.9 -3.8 flagellar motor switch protein compare
PP_4380 -0.9 -7.1 Flagellar hook-associated protein FlgL compare
PP_5090 -0.9 -2.4 putative cell division protein compare
PP_1164 -0.9 -1.2 Transcriptional regulator, LysR family compare
PP_3541 -0.9 -2.3 Transporter, MgtC family compare
PP_4263 -0.9 -2.0 putative membrane-bounded cytochrome biogenesis cycZ-like domain, copper tolerance protein compare
PP_4385 -0.9 -3.8 flagellar basal-body rod protein FlgG compare
PP_4369 -0.9 -4.7 Flagellar M-ring protein compare
PP_4394 -0.9 -3.8 flagella basal body P-ring formation protein compare
PP_2144 -0.9 -2.1 Transcriptional regulator, TetR family compare
PP_4361 -0.9 -4.0 Flagellar hook-length control protein FliK compare
PP_4098 -0.9 -1.5 UvrABC system protein C compare
PP_4354 -0.9 -2.3 flagellar export apparatus subunit compare
PP_4937 -0.9 -3.4 toluene tolerance protein compare
PP_t10 -0.9 -1.4 tRNA-Pro compare
PP_1898 -0.9 -1.4 MotA/TolQ/ExbB proton channel family protein compare
PP_5028 -0.9 -2.1 proline iminopeptidase compare
PP_5610 -0.9 -1.5 protein of unknown function compare
PP_4875 -0.9 -3.3 conserved membrane protein of unknown function compare
PP_3902 -0.9 -3.0 conserved protein of unknown function compare
PP_1317 -0.9 -2.8 Ubiquinol-cytochrome c reductase iron-sulfur subunit compare
PP_1468 -0.9 -2.3 Tyrosine recombinase XerD compare
PP_4367 -0.9 -3.0 Flagellar assembly protein FliH compare
PP_4696 -0.9 -2.8 Nitrogen regulation protein NR(I) compare
PP_4388 -0.9 -6.1 Flagellar hook protein FlgE compare
PP_4378 -0.9 -6.4 flagellin, filament structural protein compare
PP_2848 -0.9 -2.4 urease accessory protein compare
PP_1322 -0.8 -1.4 putative Lipoprotein compare
PP_2631 -0.8 -1.5 putative cellulose biosynthesis, BcsF/YhjT compare
PP_4381 -0.8 -6.0 Flagellar hook-associated protein FlgK compare
PP_0152 -0.8 -1.2 conserved exported protein of unknown function compare
PP_0048 -0.8 -1.1 conserved protein of unknown function compare
PP_4340 -0.8 -1.5 response regulator for chemotactic signal transduction compare
PP_4341 -0.8 -5.2 RNA polymerase sigma 28 (sigma F) factor for class III flagellar operons compare
PP_0872 -0.8 -3.4 Peptide chain release factor 3 compare
PP_2152 -0.8 -1.8 glycerophosphoryl diester phosphodiesterase compare
PP_1318 -0.8 -2.9 Cytochrome b compare
PP_4337 -0.8 -4.4 chemotaxis response regulator protein-glutamate methylesterase of group 1 operon compare
PP_2145 -0.8 -1.5 beta-N-acetylglucosaminidase compare
PP_3241 -0.8 -0.9 conserved exported protein of unknown function compare
PP_5093 -0.8 -1.2 twitching motility protein compare
PP_0683 -0.8 -2.5 conserved protein of unknown function compare
PP_1713 -0.8 -1.1 Transcriptional regulator PcaQ compare
PP_4581 -0.8 -2.3 putative amidotransferase compare
PP_4334 -0.8 -5.0 ParA family protein compare
PP_1862 -0.8 -2.1 conserved membrane protein of unknown function compare
PP_4382 -0.8 -4.5 Peptidoglycan hydrolase FlgJ compare
PP_4392 -0.8 -4.1 chemotaxis protein methyltransferase compare
PP_2054 -0.8 -1.5 putative DNA-binding transcriptional regulator compare
PP_4507 -0.8 -4.2 potassium transporter compare
PP_0195 -0.8 -1.3 conserved protein of unknown function compare
PP_1909 -0.8 -2.9 putative septum formation protein compare
PP_0735 -0.8 -1.3 molybdopterin-synthase adenylyltransferase compare
PP_1729 -0.8 -1.2 conserved protein of unknown function compare
PP_5337 -0.8 -3.0 LysR family transcriptional regulator compare
PP_5597 -0.8 -2.5 conserved protein of unknown function compare
PP_1674 -0.8 -2.5 5,6-dimethylbenzimidazole synthase compare
PP_1232 -0.8 -4.2 conserved exported protein of unknown function compare
PP_2371 -0.8 -2.8 Sulphite reductase hemoprotein, beta subunit compare
PP_1087 -0.8 -3.9 Outer membrane protein, OmpA family compare
PP_4384 -0.8 -4.6 Flagellar L-ring protein compare
PP_2880 -0.8 -2.1 Transcriptional regulator, TetR family compare
PP_4352 -0.8 -4.8 flagellin export apparatus, substrate specificity protein compare
PP_3634 -0.8 -3.8 putative acetyltransferase, GNAT family compare
PP_3874 -0.7 -1.3 conserved protein of unknown function compare
PP_2712 -0.7 -3.3 conserved exported protein of unknown function compare
PP_1022 -0.7 -2.0 glucose 6-phosphate-1-dehydrogenase compare
PP_4386 -0.7 -3.6 flagellar basal-body rod protein FlgF compare
PP_1629 -0.7 -2.4 Protein RecA compare
PP_4391 -0.7 -2.9 flagellar basal-body rod protein FlgB compare
PP_4215 -0.7 -3.2 pyoverdine biosynthesis related protein compare
PP_5652 -0.7 -1.6 conserved protein of unknown function, phage origin compare
PP_0711 -0.7 -1.9 putative hydrolase compare
PP_1028 -0.7 -2.5 Transcriptional regulator, LysR family compare
PP_4320 -0.7 -2.6 cytochrome c-type biogenesis protein compare
PP_4390 -0.7 -3.8 flagellar basal-body rod protein FlgC compare
PP_2067 -0.7 -2.5 putative EmrB/QacA family drug resistance transporter compare
PP_0593 -0.7 -0.7 putative ribosome-binding factor compare
PP_4150 -0.7 -2.8 microcin C transporter - ATP binding subunit compare
PP_4891 -0.7 -2.4 integral membrane ATP-dependent zinc metallopeptidase / HflK-HflC complex compare
PP_4942 -0.7 -1.4 conserved protein of unknown function compare
PP_1812 -0.7 -1.4 conserved protein of unknown function compare
PP_2442 -0.7 -1.5 conserved protein of unknown function, DUF480 family compare
PP_0002 -0.7 -3.7 probable chromosome partition protein compare
PP_4359 -0.7 -3.6 Flagellar protein FliL compare
PP_2328 -0.7 -1.9 Phosphoadenosine phosphosulfate reductase compare
PP_0630 -0.7 -1.0 DNA gyrase inhibitor YacG compare
PP_1366 -0.7 -1.0 Transcriptional regulator MvaT, P16 subunit compare
PP_5094 -0.7 -2.6 putative enzyme with PLP binding domain compare
PP_0607 -0.7 -1.0 putative Type IV pili biogenesis protein FimT compare
PP_0035 -0.7 -1.1 putative bactoprenol-linked glycose transferase compare
PP_0291 -0.7 -1.4 conserved protein of unknown function compare
PP_0289 -0.7 -2.6 Imidazoleglycerol-phosphate dehydratase compare
PP_0120 -0.7 -2.9 putative zinc ABC transporter compare
PP_0117 -0.7 -2.6 Zn(2+) ABC transporter - permease subunit compare
PP_2160 -0.7 -2.3 NADPH-dependent 7-cyano-7-deazaguanine reductase compare
PP_4015 -0.7 -3.0 HflD-like high frequency lysogenization protein compare
PP_4944 -0.7 -1.7 Carbamoyltransferase compare


Specific Phenotypes

None in this experiment

For Pseudomonas putida KT2440 in soil experiments

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=0_hrs across organisms