Experiment set23IT070 for Pseudomonas putida KT2440

Compare to:

Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs

200 most detrimental genes:

  gene name fitness t score description  
PP_1655 +2.5 7.4 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD compare
PP_1656 +2.2 9.3 ATP:GTP 3'-pyrophosphotransferase compare
PP_3726 +1.4 2.5 Enoyl-CoA hydratase/isomerase family protein compare
PP_5311 +1.2 6.6 putative signal transduction protein compare
PP_1208 +1.2 2.0 conserved protein of unknown function, SlyX family compare
PP_0168 +1.1 9.9 putative surface adhesion protein compare
PP_0167 +1.0 9.1 Toxin secretion ATP-binding protein compare
PP_4519 +1.0 7.9 Agglutination protein compare
PP_0166 +1.0 7.2 HlyD family secretion protein compare
PP_1736 +1.0 1.2 conserved protein of unknown function compare
PP_3938 +1.0 2.7 conserved protein of unknown function compare
PP_2499 +1.0 1.8 conserved protein of unknown function compare
PP_4329 +1.0 1.7 FlhB domain protein compare
PP_0916 +1.0 2.0 Amino acid transporter LysE compare
PP_4373 +1.0 6.9 Transcriptional regulator FleQ compare
PP_4196 +0.9 3.4 conserved exported protein of unknown function compare
PP_3892 +0.9 1.6 conserved protein of unknown function compare
PP_5654 +0.9 2.4 putative phosphotriesterase compare
PP_1122 +0.9 1.6 OmpA family protein compare
PP_3904 +0.9 2.1 conserved protein of unknown function compare
PP_1410 +0.9 1.3 conserved protein of unknown function compare
PP_1554 +0.9 2.2 conserved protein of unknown function compare
PP_0165 +0.8 7.0 GGDEF domain protein compare
PP_0874 +0.8 2.4 conserved protein of unknown function compare
PP_1713 +0.8 1.1 Transcriptional regulator PcaQ compare
PP_0760 +0.8 2.6 conserved membrane protein of unknown function compare
PP_3480 +0.8 3.0 conserved protein of unknown function compare
PP_1650 +0.8 5.1 Sensor protein GacS compare
PP_5717 +0.8 1.5 conserved protein of unknown function compare
PP_4246 +0.8 4.4 Periplasmic binding protein compare
PP_5648 +0.8 3.0 conserved exported protein of unknown function compare
PP_3204 +0.8 1.4 conserved protein of unknown function compare
PP_5241 +0.8 2.0 DNA-binding response regulator, LuxR family compare
PP_5552 +0.8 2.2 conserved protein of unknown function compare
PP_2306 +0.8 0.9 conserved exported protein of unknown function compare
PP_3478 +0.8 3.3 putative Secretion protein compare
PP_5423 +0.8 1.2 conserved membrane protein of unknown function compare
PP_4424 +0.8 1.1 Uncharacterized HTH-type transcriptional regulator y4tD compare
PP_0672 +0.7 5.5 Sensory box protein compare
PP_1562 +0.7 1.9 putative Phage terminase, small subunit compare
PP_4159 +0.7 2.3 Potassium-transporting ATPase C chain compare
PP_3142 +0.7 3.5 putative Sugar transferase compare
PP_5528 +0.7 1.3 conserved protein of unknown function compare
PP_3136 +0.7 2.3 putative O-acetyltransferase compare
PP_3398 +0.7 1.6 putative Curli fiber surface-exposed nucleator CsgB compare
PP_3318 +0.7 0.8 conserved protein of unknown function compare
PP_3792 +0.7 2.7 conserved protein of unknown function compare
PP_4804 +0.7 1.6 RlpA-like lipoprotein compare
PP_5471 +0.7 1.3 putative response regulator receiver protein compare
PP_0640 +0.7 2.3 conserved protein of unknown function compare
PP_4099 +0.7 3.9 two-componenent system BarA/UvrY - regulatory subunit compare
PP_4685 +0.7 3.3 conserved protein of unknown function compare
PP_2503 +0.7 1.4 putative 3-oxoacyl-(Acyl-carrier-protein) reductase compare
PP_5658 +0.7 1.7 protein of unknown function compare
PP_2540 +0.7 1.8 Oxidoreductase, short-chain dehydrogenase/reductase family compare
PP_0195 +0.7 1.4 conserved protein of unknown function compare
PP_4601 +0.7 2.9 Transcriptional regulator, LysR family compare
PP_0855 +0.7 1.4 conserved protein of unknown function compare
PP_2720 +0.7 1.2 conserved protein of unknown function compare
PP_5519 +0.7 0.9 conserved protein of unknown function of phage origin compare
PP_0414 +0.7 2.3 Polyamine ABC transporter, permease protein compare
PP_1553 +0.7 1.5 conserved protein of unknown function compare
PP_2740 +0.7 0.6 Transcriptional regulator, MerR family compare
PP_4982 +0.7 2.8 putative cytochrome b561, iron-regulated compare
PP_0319 +0.7 1.5 conserved protein of unknown function compare
PP_4930 +0.6 2.0 putative small multidrug resistance protein compare
PP_0629 +0.6 2.1 conserved membrane protein of unknown function compare
PP_2633 +0.6 1.0 conserved protein of unknown function compare
PP_5548 +0.6 1.1 protein of unknown function compare
PP_2256 +0.6 2.3 Transcriptional regulator, Cro/CI family compare
PP_1225 +0.6 2.7 7-carboxy-7-deazaguanine synthase compare
PP_4163 +0.6 2.8 conserved exported protein of unknown function compare
PP_1654 +0.6 0.6 cysteine synthase B compare
PP_0632 +0.6 2.1 leader peptidase/N-methyltransferase compare
PP_3505 +0.6 1.8 conserved protein of unknown function compare
PP_4720 +0.6 1.0 putative RNA binding protein compare
PP_3378 +0.6 1.5 putative 2-ketogluconokinase compare
PP_3117 +0.6 1.3 putative protein involved in error-prone processing of DNA lesions compare
PP_3919 +0.6 2.4 conserved protein of unknown function compare
PP_0886 +0.6 1.8 conserved exported protein of unknown function compare
PP_2825 +0.6 2.1 putative Integrase compare
PP_5473 +0.6 1.6 putative transcriptional regulator, LuxR family compare
PP_2070 +0.6 0.9 Transcriptional regulator, AraC family compare
PP_3067 +0.6 2.9 conserved protein of unknown function compare
PP_3967 +0.6 1.6 MutT/nudix family protein compare
PP_1207 +0.6 1.3 conserved protein of unknown function compare
PP_2509 +0.6 1.3 conserved protein of unknown function compare
PP_0048 +0.6 1.0 conserved protein of unknown function compare
PP_4508 +0.6 2.6 putative transcriptional regulator, AraC family compare
PP_2848 +0.6 2.3 urease accessory protein compare
PP_3083 +0.6 2.6 putative Membrane protein compare
PP_2836 +0.6 2.4 putative 2-keto-3-deoxyxylonate dehydratase compare
PP_0653 +0.6 0.8 conserved protein of unknown function compare
PP_3880 +0.6 1.1 conserved exported protein of unknown function compare
PP_5052 +0.6 1.9 tRNA(Leu) mC34,mU34 2'-O-ribose methyltransferase, AdoMet-dependent compare
PP_0108 +0.6 1.4 conserved protein of unknown function compare
PP_2581 +0.6 0.8 conserved protein of unknown function compare
PP_3441 +0.6 2.6 conserved protein of unknown function compare
PP_4156 +0.6 1.2 Transcriptional regulator, LysR family compare
PP_5022 +0.6 1.3 Glutamine transport ATP-binding protein GlnQ compare
PP_2054 +0.6 1.1 putative DNA-binding transcriptional regulator compare
PP_5255 +0.6 2.3 putative hydrolase compare
PP_3140 +0.6 2.7 Glycosyl transferase, group 2 family protein compare
PP_3103 +0.6 2.9 conserved protein of unknown function compare
PP_0703 +0.6 2.1 putative Transmembrane sensor compare
PP_4541 +0.6 1.9 peptidyl-prolyl cis-trans isomerase A (rotamase A) compare
PP_3328 +0.6 1.6 putative Ring-cleaving dioxygenase compare
PP_2899 +0.6 0.7 conserved exported protein of unknown function compare
PP_1605 +0.6 1.8 Ribonuclease HII compare
PP_3492 +0.6 2.9 isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) compare
PP_3173 +0.6 2.6 N-ethylmaleimide reductase, FMN-linked compare
PP_1456 +0.5 2.5 conserved membrane protein of unknown function compare
PP_1692 +0.5 1.2 conserved protein of unknown function compare
PP_4266 +0.5 1.1 Adenine phosphoribosyltransferase compare
PP_0929 +0.5 2.1 putative Lipoprotein compare
PP_5144 +0.5 2.3 conserved protein of unknown function compare
PP_0511 +0.5 2.2 putative methyltransferase compare
PP_4423 +0.5 2.7 conserved protein of unknown function compare
PP_1020 +0.5 1.2 putative glucose-6-phosphate 1-epimerase compare
PP_3392 +0.5 0.6 conserved protein of unknown function compare
PP_2322 +0.5 2.3 Major outer membrane lipoprotein compare
PP_0307 +0.5 1.4 conserved exported protein of unknown function compare
PP_4986 +0.5 2.2 Channel protein, hemolysin III family compare
PP_3105 +0.5 0.9 conserved membrane protein of unknown function compare
PP_2705 +0.5 1.6 conserved protein of unknown function compare
PP_2055 +0.5 0.8 putative isomerase compare
PP_2996 +0.5 2.2 conserved protein of unknown function compare
PP_3662 +0.5 2.2 conserved protein of unknown function compare
PP_3477 +0.5 2.0 Putative secretion type II protein related to protein G compare
PP_4710 +0.5 3.2 tRNA pseudouridine 55 synthase compare
PP_3138 +0.5 2.2 VirK domain protein compare
PP_2474 +0.5 1.7 Glutathione S-transferase family protein compare
PP_5695 +0.5 1.9 conserved protein of unknown function compare
PP_3899 +0.5 1.5 conserved protein of unknown function, hicB family compare
PP_4015 +0.5 2.5 HflD-like high frequency lysogenization protein compare
PP_4030 +0.5 2.0 Enoyl-CoA hydratase/isomerase family protein compare
PP_2970 +0.5 1.0 conserved exported protein of unknown function compare
PP_1190 +0.5 1.9 conserved protein of unknown function compare
PP_2654 +0.5 2.0 putative Glutathione S-transferase compare
PP_0504 +0.5 1.7 Outer membrane protein OprG compare
PP_1825 +0.5 1.2 conserved exported protein of unknown function compare
PP_1303 +0.5 2.1 Sulfate adenylyltransferase subunit 2 compare
PP_1982 +0.5 1.5 small heat shock protein IbpA compare
PP_1310 +0.5 1.4 conserved hypothetical protein compare
PP_3935 +0.5 1.6 conserved protein of unknown function compare
PP_1951 +0.5 2.4 Oxidoreductase, short chain dehydrogenase/reductase family compare
PP_2879 +0.5 2.7 conserved exported protein of unknown function compare
PP_3280 +0.5 1.5 3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase compare
PP_5556 +0.5 1.5 putative bacteriophage lysis protein compare
PP_4213 +0.5 1.3 putative dipeptidase compare
PP_4782 +0.5 1.7 Phosphomethylpyrimidine kinase compare
PP_2175 +0.5 2.0 putative 3-oxoacyl-(Acyl-carrier-protein) reductase compare
PP_1647 +0.5 1.2 conserved protein of unknown function compare
PP_0685 +0.5 0.6 conserved protein of unknown function compare
PP_0834 +0.5 0.8 Sec translocation complex subunit YajC compare
PP_3094 +0.5 1.3 conserved exported protein of unknown function compare
PP_5713 +0.5 2.5 conserved protein of unknown function compare
PP_3749 +0.5 1.8 conserved protein of unknown function compare
PP_4907 +0.5 1.8 Thiosulfate sulfurtransferase compare
PP_5017 +0.5 0.6 Sec-independent protein translocase protein TatB compare
PP_2121 +0.5 0.7 putative Lipoprotein compare
PP_2087 +0.5 0.8 CmpX protein compare
PP_1074 +0.5 1.2 DNA-binding transcriptional repressor - GlpR-glycerol-3-phosphate compare
PP_3685 +0.5 1.2 conserved protein of unknown function compare
PP_5586 +0.5 1.6 exported protein of unknown function compare
PP_3482 +0.5 2.8 conserved protein of unknown function compare
PP_2835 +0.5 1.0 putative oxidoreductase compare
PP_3589 +0.5 2.1 serine:H+ symport permease, threonine-insensitive compare
PP_5610 +0.5 1.1 protein of unknown function compare
PP_0900 +0.5 1.2 PAP2 family protein compare
PP_5339 +0.5 1.1 transcriptional regulator, ornithine utilization compare
PP_1587 +0.5 2.6 NhaP-type Na+/H+ and K+/H+ antiporters compare
PP_4178 +0.5 1.7 Dienelactone hydrolase family protein compare
PP_3365 +0.5 0.7 Acetolactate synthase compare
PP_4768 +0.5 1.1 Exonuclease compare
PP_4848 +0.5 0.9 Curved DNA-binding protein compare
PP_0989 +0.5 0.9 glycine cleavage system H protein 1 compare
PP_3131 +0.5 3.1 conserved membrane protein of unknown function compare
PP_1756 +0.5 1.9 conserved exported protein of unknown function compare
PP_3645 +0.5 2.0 putative L-proline amide hydrolase compare
PP_5066 +0.5 1.6 K(+)/H(+) antiporter NhaP2 compare
PP_0880 +0.5 1.3 dipeptide ABC transporter - putative membrane subunit compare
PP_5451 +0.5 1.4 protein of unknown function compare
PP_5687 +0.5 1.5 putative permease of the major facilitator superfamily compare
PP_5096 +0.5 1.6 putative factor involved in shape determination compare
PP_4624 +0.5 2.8 putative hydrolase, alpha/beta fold family compare
PP_2659 +0.5 2.3 phosphate ABC transporter ATP-binding protein compare
PP_1191 +0.5 1.7 S4 domain protein compare
PP_3444 +0.5 1.5 Glyoxalase family protein compare
PP_2388 +0.5 1.3 Transporter, LysE family compare
PP_4771 +0.5 1.1 conserved membrane protein of unknown function compare
PP_0865 +0.5 1.5 ECF subfamily RNA polymerase sigma-24 factor compare
PP_0303 +0.5 1.4 dehydrocarnitine cleavage enzyme compare
PP_0355 +0.5 1.9 Response regulator compare
PP_5059 +0.5 1.8 conserved exported protein of unknown function compare
PP_5265 +0.4 1.5 Xanthine phosphoribosyltransferase compare
PP_3913 +0.4 2.1 protein of unknown function compare
PP_3371 +0.4 1.7 Sensor histidine kinase compare
PP_0859 +0.4 2.0 putative ketoglutaramate omega-amidase compare
PP_4959 +0.4 3.2 diguanylate cyclase compare


Specific Phenotypes

For 2 genes in this experiment

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs in Pseudomonas putida KT2440

For soil Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=nonsterile; Collection=outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=144_hrs across organisms