Experiment set22IT096 for Pseudomonas putida KT2440

Growth at 10C; with MOPS

Group: temperature
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM), pH=7
Culturing: Putida_ML5_PNNL, 24-well transparent microplate; Multitron, Aerobic, at 10 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 4 genes in this experiment

For temperature D-Glucose in Pseudomonas putida KT2440

For temperature D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Glycogen metabolism	1
Orphan regulatory proteins	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycogen biosynthesis I (from ADP-D-Glucose)	4	3	1
starch biosynthesis	10	5	1