Experiment set22IT091 for Pseudomonas simiae WCS417

R2A with Polymyxin B sulfate 0.003 mg/ml

Group: stress
Media: R2A + Polymyxin B sulfate (0.003 mg/ml)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 12 genes in this experiment

For stress Polymyxin B sulfate in Pseudomonas simiae WCS417

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Orphan regulatory proteins	2
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
Arginine Biosynthesis extended	1
Beta-lactamase	1
Multidrug Resistance Efflux Pumps	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-arginine biosynthesis II (acetyl cycle)	10	10	2
L-ornithine biosynthesis I	5	5	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
superpathway of arginine and polyamine biosynthesis	17	15	1