Experiment set22IT090 for Pseudomonas simiae WCS417

Compare to:

R2A with Polymyxin B sulfate 0.003 mg/ml

Group: stress
Media: R2A + Polymyxin B sulfate (0.003 mg/ml)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 12 genes in this experiment

For stress Polymyxin B sulfate in Pseudomonas simiae WCS417

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 1
Acid resistance mechanisms 1
Arginine and Ornithine Degradation 1
Beta-lactamase 1
Choline and Betaine Uptake and Betaine Biosynthesis 1
Orphan regulatory proteins 1
Oxidative stress 1
Polyamine Metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
arginine dependent acid resistance 1 1 1
choline degradation I 2 2 1
glycine betaine biosynthesis II (Gram-positive bacteria) 2 2 1
glycine betaine biosynthesis I (Gram-negative bacteria) 2 2 1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway) 2 2 1
putrescine biosynthesis I 2 2 1
β-alanine biosynthesis IV 2 1 1
β-alanine biosynthesis I 2 1 1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway) 3 3 1
putrescine biosynthesis II 3 3 1
choline-O-sulfate degradation 3 3 1
glycine betaine biosynthesis III (plants) 3 2 1
dimethylsulfoniopropanoate biosynthesis I (Wollastonia) 3 1 1
superpathway of putrescine biosynthesis 4 4 1
choline degradation IV 4 2 1
spermidine biosynthesis III 4 1 1
dimethylsulfoniopropanoate biosynthesis II (Spartina) 4 1 1
superpathway of polyamine biosynthesis I 8 6 1
superpathway of polyamine biosynthesis II 8 6 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 8 1
superpathway of L-arginine and L-ornithine degradation 13 10 1
superpathway of arginine and polyamine biosynthesis 17 15 1