Experiment set22IT087 for Pseudomonas simiae WCS417

R2A with Polymyxin B sulfate 0.002 mg/ml

Group: stress
Media: R2A + Polymyxin B sulfate (0.002 mg/ml)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 6 genes in this experiment

For stress Polymyxin B sulfate in Pseudomonas simiae WCS417

For stress Polymyxin B sulfate across organisms

SEED Subsystems

Subsystem	#Specific
Acid resistance mechanisms	1
Arginine and Ornithine Degradation	1
Beta-lactamase	1
Orphan regulatory proteins	1
Polyamine Metabolism	1
Proteasome bacterial	1
Proteolysis in bacteria, ATP-dependent	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Arginine and proline metabolism
• Peptidoglycan biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arginine dependent acid resistance	1	1	1
putrescine biosynthesis I	2	2	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
L-arginine degradation III (arginine decarboxylase/agmatinase pathway)	2	2	1
putrescine biosynthesis II	3	3	1
L-arginine degradation IV (arginine decarboxylase/agmatine deiminase pathway)	3	3	1
superpathway of putrescine biosynthesis	4	4	1
spermidine biosynthesis III	4	1	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
superpathway of polyamine biosynthesis II	8	6	1
superpathway of polyamine biosynthesis I	8	6	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	8	1
superpathway of L-arginine and L-ornithine degradation	13	10	1
superpathway of arginine and polyamine biosynthesis	17	15	1