Experiment set22IT083 for Pseudomonas simiae WCS417
R2A with Polymyxin B sulfate 0.002 mg/ml
Group: stressMedia: R2A + Polymyxin B sulfate (0.002 mg/ml)
Culturing: fluoroDangl_ML3, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel on 28-Jun-22
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate
Specific Phenotypes
For 9 genes in this experiment
For stress Polymyxin B sulfate in Pseudomonas simiae WCS417
For stress Polymyxin B sulfate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Arginine and Ornithine Degradation | 1 |
| Beta-lactamase | 1 |
| Hemin transport system | 1 |
| Orphan regulatory proteins | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Urea cycle and metabolism of amino groups
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-arginine degradation VII (arginase 3 pathway) | 2 | 2 | 1 |
| putrescine biosynthesis III | 2 | 2 | 1 |
| L-canavanine degradation I | 2 | 1 | 1 |
| L-arginine degradation I (arginase pathway) | 3 | 3 | 1 |
| superpathway of putrescine biosynthesis | 4 | 4 | 1 |
| L-arginine degradation VI (arginase 2 pathway) | 4 | 4 | 1 |
| urea cycle | 5 | 4 | 1 |
| L-Nδ-acetylornithine biosynthesis | 7 | 6 | 1 |
| L-citrulline biosynthesis | 8 | 8 | 1 |
| superpathway of polyamine biosynthesis I | 8 | 6 | 1 |
| superpathway of polyamine biosynthesis II | 8 | 6 | 1 |
| superpathway of L-citrulline metabolism | 12 | 10 | 1 |
| superpathway of hyoscyamine (atropine) and scopolamine biosynthesis | 16 | 4 | 1 |
| superpathway of arginine and polyamine biosynthesis | 17 | 15 | 1 |