Experiment set22IT076 for Escherichia coli BW25113

T7_phage 0.0000015625 MOI TC5

Group: phage
Media: LB_plus_SM_buffer + T7_phage (1.56E-06 MOI)
Culturing: Keio_ML9a, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Crystal on 21-Aug-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 3 genes in this experiment

For phage T7_phage in Escherichia coli BW25113

For phage T7_phage across organisms

SEED Subsystems

Subsystem	#Specific
Rhamnose containing glycans	2
dTDP-rhamnose synthesis	2
linker unit-arabinogalactan synthesis	2
Capsular heptose biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Streptomycin biosynthesis
• Polyketide sugar unit biosynthesis
• Lipopolysaccharide biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
dTDP-β-L-rhamnose biosynthesis	5	5	2
dTDP-4-O-demethyl-β-L-noviose biosynthesis	5	3	1
O-antigen building blocks biosynthesis (E. coli)	11	11	2
dTDP-sibirosamine biosynthesis	6	3	1
dTDP-L-daunosamine biosynthesis	6	3	1
superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis	19	7	2
lipid A-core biosynthesis (E. coli K-12)	10	9	1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis	33	14	2
superpathway of novobiocin biosynthesis	19	4	1
superpathway of Kdo2-lipid A biosynthesis	25	24	1