Experiment set22IT071 for Escherichia coli BW25113
T7_phage 0.000015625 MOI TC4
Group: phageMedia: LB_plus_SM_buffer + T7_phage (0.000015625 MOI)
Culturing: Keio_ML9a, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Crystal on 21-Aug-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 2 genes in this experiment
For phage T7_phage in Escherichia coli BW25113
For phage T7_phage across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Rhamnose containing glycans | 2 |
| dTDP-rhamnose synthesis | 2 |
| linker unit-arabinogalactan synthesis | 2 |
| Capsular heptose biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| dTDP-β-L-rhamnose biosynthesis | 5 | 5 | 2 |
| dTDP-4-O-demethyl-β-L-noviose biosynthesis | 5 | 3 | 1 |
| O-antigen building blocks biosynthesis (E. coli) | 11 | 11 | 2 |
| dTDP-sibirosamine biosynthesis | 6 | 3 | 1 |
| dTDP-L-daunosamine biosynthesis | 6 | 3 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 7 | 2 |
| superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis | 33 | 14 | 2 |
| superpathway of novobiocin biosynthesis | 19 | 4 | 1 |