Experiment set22IT052 for Pseudomonas putida KT2440

Growth with Sodium Chloride 500 mM

Group: stress
Media: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + Sodium Chloride (500 mM)
Culturing: Putida_ML5_PNNL, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20

Specific Phenotypes

For 7 genes in this experiment

For stress D-Glucose in Pseudomonas putida KT2440

For stress D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
D-gluconate and ketogluconates metabolism	2
Entner-Doudoroff Pathway	1
Fermentations: Mixed acid	1
Mannitol Utilization	1
Pyruvate metabolism I: anaplerotic reactions, PEP	1
Respiratory dehydrogenases 1	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Fructose and mannose metabolism
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Carbon fixation in photosynthetic organisms
• Reductive carboxylate cycle (CO2 fixation)
• Retinol metabolism
• Limonene and pinene degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
mannitol degradation I	1	1	1
CO2 fixation into oxaloacetate (anaplerotic)	2	2	1
glucose degradation (oxidative)	5	5	1
glucose and glucose-1-phosphate degradation	5	4	1
mannitol cycle	5	2	1
L-ascorbate biosynthesis VIII (engineered pathway)	7	5	1
C4 photosynthetic carbon assimilation cycle, NADP-ME type	7	4	1
partial TCA cycle (obligate autotrophs)	8	8	1
nitrogen remobilization from senescing leaves	8	6	1
C4 photosynthetic carbon assimilation cycle, NAD-ME type	11	7	1
formaldehyde assimilation I (serine pathway)	13	7	1
C4 photosynthetic carbon assimilation cycle, PEPCK type	14	8	1
mixed acid fermentation	16	12	1
superpathway of hexitol degradation (bacteria)	18	13	1
gluconeogenesis II (Methanobacterium thermoautotrophicum)	18	9	1
hexitol fermentation to lactate, formate, ethanol and acetate	19	14	1
ethene biosynthesis V (engineered)	25	18	1
Methanobacterium thermoautotrophicum biosynthetic metabolism	56	21	1