Experiment set22IT010 for Escherichia coli BW25113

N4_phage 0.009375 MOI TC2

Group: phage
Media: LB_plus_SM_buffer + N4_phage (0.009375 MOI)
Culturing: Keio_ML9a, 48 well microplate; Tecan Infinite F200, Aerobic, at 37 (C), shaken=orbital
By: Crystal on 24-Jul-17
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 12 genes in this experiment

For phage N4_phage in Escherichia coli BW25113

For phage N4_phage across organisms

SEED Subsystems

Subsystem	#Specific
Biotin biosynthesis	1
LOS core oligosaccharide biosynthesis	1
Orphan regulatory proteins	1
Ribosome LSU bacterial	1
n-Phenylalkanoic acid degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Starch and sucrose metabolism
• Lipopolysaccharide biosynthesis
• Lipoic acid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
long-chain fatty acid activation	1	1	1
cinnamoyl-CoA biosynthesis	2	1	1
γ-linolenate biosynthesis II (animals)	2	1	1
linoleate biosynthesis II (animals)	2	1	1
3-methyl-branched fatty acid α-oxidation	6	3	2
alkane biosynthesis II	3	1	1
oleate biosynthesis I (plants)	3	1	1
sucrose degradation IV (sucrose phosphorylase)	4	4	1
phytol degradation	4	3	1
phosphatidylcholine acyl editing	4	2	1
pinosylvin metabolism	4	1	1
wax esters biosynthesis II	4	1	1
long chain fatty acid ester synthesis (engineered)	4	1	1
sporopollenin precursors biosynthesis	18	5	4
5,6-dehydrokavain biosynthesis (engineered)	10	8	2
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
octane oxidation	5	2	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	2	1
fatty acid salvage	6	5	1
stearate biosynthesis II (bacteria and plants)	6	5	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	2	1
stearate biosynthesis I (animals)	6	1	1
ceramide degradation by α-oxidation	7	2	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
2-deoxy-D-ribose degradation II	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	4	1
3-phenylpropanoate degradation	10	4	1
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	2	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
palmitate biosynthesis III	29	21	1
oleate β-oxidation	35	32	1