Experiment set22IT009 for Pseudomonas putida KT2440
Growth with Sodium Chloride 500 mM; with MOPS
Group: stressMedia: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + 3-(N-morpholino)propanesulfonic acid (40 mM) + Sodium Chloride (500 mM), pH=7
Culturing: Putida_ML5_PNNL, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20
Specific Phenotypes
For 8 genes in this experiment
For stress D-Glucose in Pseudomonas putida KT2440
For stress D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-gluconate and ketogluconates metabolism | 2 |
| Entner-Doudoroff Pathway | 1 |
| Mannitol Utilization | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Fructose and mannose metabolism
- gamma-Hexachlorocyclohexane degradation
- Bisphenol A degradation
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Retinol metabolism
- Limonene and pinene degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| mannitol degradation I | 1 | 1 | 1 |
| glucose degradation (oxidative) | 5 | 5 | 1 |
| glucose and glucose-1-phosphate degradation | 5 | 4 | 1 |
| mannitol cycle | 5 | 2 | 1 |
| L-ascorbate biosynthesis VIII (engineered pathway) | 7 | 5 | 1 |
| superpathway of hexitol degradation (bacteria) | 18 | 13 | 1 |
| hexitol fermentation to lactate, formate, ethanol and acetate | 19 | 14 | 1 |