Experiment set21IT095 for Escherichia coli BW25113

Compare to:

M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin

Group: nutrient
Media: M9_Thauers_noBiotin + Avidin (0.02 mg/mL)
Culturing: KEIO_ML9a, flask, Aerobic, at 37 (C), shaken=200 rpm
By: Valentine on 7-Jul-17
Media components: 2 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 6.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride, Thauer's vitamin mix no Biotin (0.02 mg/L Pyridoxine HCl, 0.01 mg/L 4-Aminobenzoic acid, 0.01 mg/L Lipoic acid, 0.01 mg/L Nicotinic Acid, 0.01 mg/L Riboflavin, 0.01 mg/L Thiamine HCl, 0.01 mg/L calcium pantothenate, 0.004 mg/L Folic Acid, 0.0002 mg/L Cyanocobalamin, 0.4 mg/L Choline chloride)

Specific Phenotypes

For 4 genes in this experiment

For nutrient Avidin in Escherichia coli BW25113

For nutrient Avidin across organisms

SEED Subsystems

Subsystem #Specific
Ammonia assimilation 2
Biotin biosynthesis 2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamate biosynthesis I 2 2 2
L-glutamine degradation I 1 1 1
L-glutamine degradation II 1 1 1
ammonia assimilation cycle III 3 3 2
8-amino-7-oxononanoate biosynthesis III 2 1 1
8-amino-7-oxononanoate biosynthesis II 2 1 1
L-glutamate and L-glutamine biosynthesis 7 5 2
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 1
L-asparagine biosynthesis III (tRNA-dependent) 4 1 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 1 1
8-amino-7-oxononanoate biosynthesis IV 5 4 1
biotin biosynthesis II 6 4 1
biotin biosynthesis I 15 15 2
L-citrulline biosynthesis 8 6 1
8-amino-7-oxononanoate biosynthesis I 11 11 1
superpathway of L-citrulline metabolism 12 8 1