Experiment set21IT094 for Escherichia coli BW25113
M9 Glucose 0.2%, Thauer's vit mix without biotin with Avidin
Group: nutrientMedia: M9_Thauers_noBiotin + Avidin (0.02 mg/mL)
Culturing: KEIO_ML9a, flask, Aerobic, at 37 (C), shaken=200 rpm
By: Valentine on 7-Jul-17
Media components: 2 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 6.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride, Thauer's vitamin mix no Biotin (0.02 mg/L Pyridoxine HCl, 0.01 mg/L 4-Aminobenzoic acid, 0.01 mg/L Lipoic acid, 0.01 mg/L Nicotinic Acid, 0.01 mg/L Riboflavin, 0.01 mg/L Thiamine HCl, 0.01 mg/L calcium pantothenate, 0.004 mg/L Folic Acid, 0.0002 mg/L Cyanocobalamin, 0.4 mg/L Choline chloride)
Specific Phenotypes
For 4 genes in this experiment
For nutrient Avidin in Escherichia coli BW25113
For nutrient Avidin across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Biotin biosynthesis | 3 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| biotin biosynthesis from 8-amino-7-oxononanoate I | 4 | 4 | 3 |
| biotin biosynthesis from 8-amino-7-oxononanoate II | 4 | 3 | 2 |
| biotin biosynthesis II | 6 | 4 | 3 |
| 8-amino-7-oxononanoate biosynthesis III | 2 | 1 | 1 |
| 8-amino-7-oxononanoate biosynthesis II | 2 | 1 | 1 |
| biotin biosynthesis from 8-amino-7-oxononanoate III | 5 | 3 | 2 |
| biotin biosynthesis I | 15 | 15 | 5 |
| 8-amino-7-oxononanoate biosynthesis IV | 5 | 4 | 1 |
| 8-amino-7-oxononanoate biosynthesis I | 11 | 11 | 2 |