Experiment set21IT089 for Escherichia coli BW25113

M9 Glucose 0.2%, Thauer's vit mix

Group: nutrient
Media: M9_Thauers
Culturing: KEIO_ML9a, flask, Aerobic, at 37 (C), shaken=200 rpm
By: Valentine on 7-Jul-17
Media components: 2 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 6.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride, 1 g/L Ammonium chloride, Thauer's vitamin mix (0.02 mg/L Pyridoxine HCl, 0.01 mg/L 4-Aminobenzoic acid, 0.01 mg/L Lipoic acid, 0.01 mg/L Nicotinic Acid, 0.01 mg/L Riboflavin, 0.01 mg/L Thiamine HCl, 0.01 mg/L calcium pantothenate, 0.004 mg/L biotin, 0.004 mg/L Folic Acid, 0.0002 mg/L Cyanocobalamin, 0.4 mg/L Choline chloride)

Specific Phenotypes

For 2 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Acetoin, butanediol metabolism	1
Ammonia assimilation	1
Branched-Chain Amino Acid Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine biosynthesis
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyruvate fermentation to (R)-acetoin II	2	1	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
L-valine biosynthesis	4	4	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
superpathway of (R,R)-butanediol biosynthesis	5	2	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of L-threonine metabolism	18	16	1