Experiment set20S609 for Agrobacterium fabrum C58

Phage 100.2 + bactiera replicate 3

Group: phage
Media: LB_plus_SM_buffer
Culturing: Agro_ML11a, shaking in plate reader, aerobic, at 30C (C), shaken=double orbital, continuous, 205cpm, (yes)
By: James on 9/13/2023
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride

Specific Phenotypes

For 6 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Anaerobic respiratory reductases	1
Arsenic resistance	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Heat shock dnaK gene cluster extended	1
MLST	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycerolipid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
arsenate detoxification III	2	1	1
glycerol degradation I	3	2	1
glycerol and glycerophosphodiester degradation	4	3	1
arsenic detoxification (bacteria)	4	2	1
arsenate detoxification I	6	3	1
arsenic detoxification (plants)	6	2	1
arsenic detoxification (yeast)	12	3	1