Experiment set20IT018 for Escherichia coli BW25113

M9 Glucose 2%, 20 mM NO3- anaerobic

Group:
Media: M9_Avi_noCarbon + D-Glucose (111 mM) + Sodium nitrate (20 mM)
Culturing: Keio_ML9a, tube, Anaerobic, at 37 (C), shaken=250 rpm
By: Avi on 27-Apr-17

Specific Phenotypes

For 29 genes in this experiment

For D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Arginine Biosynthesis extended	1
Beta-Glucoside Metabolism	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
LOS core oligosaccharide biosynthesis	1
Lysine Biosynthesis DAP Pathway	1
Molybdenum cofactor biosynthesis	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
NAD and NADP cofactor biosynthesis global	1
Orphan regulatory proteins	1
PnuC-like transporters	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Lysine biosynthesis
• Arginine and proline metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Urea cycle and metabolism of amino groups
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Lipoic acid metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Phosphatidylinositol signaling system

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
chitobiose degradation	2	2	1
CDP-diacylglycerol biosynthesis II	4	4	1
CDP-diacylglycerol biosynthesis I	4	4	1
L-arginine degradation II (AST pathway)	5	5	1
L-ornithine biosynthesis I	5	5	1
CDP-diacylglycerol biosynthesis III	5	3	1
phosphatidylglycerol biosynthesis I	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
molybdopterin biosynthesis	6	6	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-lysine biosynthesis I	9	9	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	8	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	1
superpathway of arginine and polyamine biosynthesis	17	16	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	1
aspartate superpathway	25	24	1
superpathway of phospholipid biosynthesis II (plants)	28	10	1