Experiment set20IT012 for Pseudomonas putida KT2440
Growth at pH9 and carbon source D-Glucose
Group: pHMedia: soilextract_PNNL_Prosser_PlotA_B_20191220 + D-Glucose (10 mM) + Ammonium chloride (10 mM) + AMPSO (40 mM), pH=9
Culturing: Putida_ML5_PNNL, 96 deep-well microplate; 1 mL volume, Aerobic, at 30 (C)
By: Joshua Elmore on 1/6/20
Specific Phenotypes
For 5 genes in this experiment
For pH D-Glucose in Pseudomonas putida KT2440
For pH D-Glucose across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
D-gluconate and ketogluconates metabolism | 1 |
Entner-Doudoroff Pathway | 1 |
Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- gamma-Hexachlorocyclohexane degradation
- Bisphenol A degradation
- 1- and 2-Methylnaphthalene degradation
- Naphthalene and anthracene degradation
- Benzoate degradation via CoA ligation
- Ethylbenzene degradation
- Retinol metabolism
- Limonene and pinene degradation
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
glucose degradation (oxidative) | 5 | 5 | 1 |
glucose and glucose-1-phosphate degradation | 5 | 4 | 1 |
L-ascorbate biosynthesis VIII (engineered pathway) | 7 | 5 | 1 |