Experiment set20IT012 for Escherichia coli BW25113

M9 Glucose 2% aerobic

Group:
Media: M9_Avi_noCarbon + D-Glucose (111 mM)
Culturing: Keio_ML9a, tube, Aerobic, at 37 (C), shaken=250 rpm
By: Avi on 27-Apr-17

Specific Phenotypes

For 26 genes in this experiment

For D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Branched-Chain Amino Acid Biosynthesis	2
Acetoin, butanediol metabolism	1
Alanine biosynthesis	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
LOS core oligosaccharide biosynthesis	1
N-Acetyl-Galactosamine and Galactosamine Utilization	1
NAD and NADP cofactor biosynthesis global	1
Orphan regulatory proteins	1
PnuC-like transporters	1
Pyruvate Alanine Serine Interconversions	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Valine, leucine and isoleucine biosynthesis
• Lipopolysaccharide biosynthesis
• Glycerophospholipid metabolism
• Butanoate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Ascorbate and aldarate metabolism
• Fatty acid biosynthesis
• Lysine degradation
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Lipoic acid metabolism
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from histidine and purine
• Phosphatidylinositol signaling system

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine biosynthesis I	2	2	1
chitobiose degradation	2	2	1
pyruvate fermentation to (R)-acetoin II	2	1	1
pyruvate fermentation to (S)-acetoin	3	3	1
pyruvate fermentation to (R)-acetoin I	3	2	1
L-valine biosynthesis	4	4	1
CDP-diacylglycerol biosynthesis II	4	4	1
CDP-diacylglycerol biosynthesis I	4	4	1
superpathway of L-alanine biosynthesis	4	4	1
pyruvate fermentation to isobutanol (engineered)	5	4	1
CDP-diacylglycerol biosynthesis III	5	3	1
superpathway of (R,R)-butanediol biosynthesis	5	2	1
phosphatidylglycerol biosynthesis II	6	6	1
phosphatidylglycerol biosynthesis I	6	6	1
L-isoleucine biosynthesis IV	6	4	1
superpathway of 2,3-butanediol biosynthesis	6	3	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
L-isoleucine biosynthesis II	8	4	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of phospholipid biosynthesis III (E. coli)	12	12	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of cardiolipin biosynthesis (bacteria)	13	11	1
type I lipoteichoic acid biosynthesis (S. aureus)	17	5	1
superpathway of L-threonine metabolism	18	16	1
superpathway of phospholipid biosynthesis II (plants)	28	10	1