Experiment set1S90 for Brevundimonas sp. GW460-12-10-14-LB2

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D-Glucose carbon source; 30C

Group: carbon source
Media: RCH2_defined_noCarbon + D-Glucose (20 mM)
Culturing: Brev2_ML6a, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Hira on 1-Mar-23
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 39 genes in this experiment

For carbon source D-Glucose in Brevundimonas sp. GW460-12-10-14-LB2

For carbon source D-Glucose across organisms

SEED Subsystems

Subsystem #Specific
Cysteine Biosynthesis 2
Glycine and Serine Utilization 2
Methionine Biosynthesis 2
Methionine Degradation 2
Terminal cytochrome O ubiquinol oxidase 2
Terminal cytochrome oxidases 2
Acetyl-CoA fermentation to Butyrate 1
Archaeal lipids 1
Aromatic amino acid degradation 1
Biogenesis of c-type cytochromes 1
Carotenoids 1
Conenzyme B12 related Hypothetical: Clusters with cobST 1
Copper homeostasis 1
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Glutathione: Non-redox reactions 1
Glutathione: Redox cycle 1
Isoprenoid Biosynthesis 1
Maltose and Maltodextrin Utilization 1
Methylglyoxal Metabolism 1
NAD regulation 1
Polyhydroxybutyrate metabolism 1
Polyprenyl Diphosphate Biosynthesis 1
Pterin biosynthesis 1
RNA processing and degradation, bacterial 1
Serine-glyoxylate cycle 1
Utilization of glutathione as a sulphur source 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
hydrogen sulfide biosynthesis II (mammalian) 6 6 6
L-cysteine degradation II 3 3 3
L-cysteine biosynthesis III (from L-homocysteine) 2 2 2
trans, trans-farnesyl diphosphate biosynthesis 2 2 2
L-tyrosine biosynthesis IV 1 1 1
geranylgeranyl diphosphate biosynthesis 1 1 1
L-phenylalanine degradation I (aerobic) 1 1 1
octaprenyl diphosphate biosynthesis 1 1 1
geranyl diphosphate biosynthesis 1 1 1
D-serine degradation 3 3 2
L-tryptophan degradation II (via pyruvate) 3 2 2
L-serine degradation 3 2 2
L-methionine degradation II 3 2 2
polyisoprenoid biosynthesis (E. coli) 5 4 3
homocysteine and cysteine interconversion 4 3 2
all-trans-farnesol biosynthesis 4 3 2
methyl phomopsenoate biosynthesis 4 2 2
(3R)-linalool biosynthesis 2 1 1
linalool biosynthesis I 2 1 1
(3S)-linalool biosynthesis 2 1 1
glutathione degradation (DUG pathway) 2 1 1
sulfoquinovosyl diacylglycerol biosynthesis 2 1 1
methylglyoxal degradation III 2 1 1
superpathway of L-cysteine biosynthesis (mammalian) 5 4 2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
stellatic acid biosynthesis 8 3 3
L-methionine biosynthesis II 6 4 2
superpathway of L-cysteine biosynthesis (fungi) 6 4 2
L-phenylalanine degradation V 3 2 1
methylglyoxal degradation I 3 2 1
methylglyoxal degradation VIII 3 2 1
glutathione-peroxide redox reactions 3 2 1
polyhydroxybutanoate biosynthesis 3 2 1
bisabolene biosynthesis (engineered) 6 3 2
L-threonine degradation I 6 3 2
taurine biosynthesis III 3 1 1
superpathway of linalool biosynthesis 3 1 1
starch degradation I 3 1 1
plaunotol biosynthesis 3 1 1
ophiobolin F biosynthesis 3 1 1
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) 10 5 3
L-isoleucine biosynthesis I (from threonine) 7 7 2
glycine betaine degradation III 7 3 2
superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) 12 12 3
superpathway of methylglyoxal degradation 8 5 2
superpathway of ubiquinol-8 biosynthesis (early decarboxylation) 12 7 3
L-mimosine degradation 8 4 2
(2S)-ethylmalonyl-CoA biosynthesis 4 2 1
glycine betaine degradation I 8 3 2
glutathione-mediated detoxification I 8 3 2
ipsdienol biosynthesis 4 1 1
taxadiene biosynthesis (engineered) 13 12 3
hypoglycin biosynthesis 14 4 3
tRNA processing 10 8 2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) 10 8 2
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 1
mono-trans, poly-cis decaprenyl phosphate biosynthesis 5 2 1
peptido-conjugates in tissue regeneration biosynthesis 17 6 3
γ-glutamyl cycle 6 3 1
leukotriene biosynthesis 6 2 1
paspaline biosynthesis 6 1 1
superpathway of L-isoleucine biosynthesis I 13 12 2
L-cysteine biosynthesis VI (reverse transsulfuration) 7 4 1
acetyl-CoA fermentation to butanoate 7 3 1
brassicicene C biosynthesis 7 1 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 11 2
superpathway of L-methionine salvage and degradation 16 9 2
glycogen degradation I 8 3 1
fusicoccin A biosynthesis 8 1 1
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of L-threonine metabolism 18 11 2
superpathway of menaquinol-8 biosynthesis III 9 2 1
4-oxopentanoate degradation 9 2 1
viridicatumtoxin biosynthesis 9 1 1
superpathway of demethylmenaquinol-8 biosynthesis I 9 1 1
superpathway of menaquinol-8 biosynthesis II 10 2 1
superpathway of menaquinol-8 biosynthesis I 10 2 1
detoxification of reactive carbonyls in chloroplasts 10 1 1
ethylmalonyl-CoA pathway 11 3 1
purine nucleobases degradation II (anaerobic) 24 7 2
superpathway of ergosterol biosynthesis II 26 11 2
superpathway of ergosterol biosynthesis I 26 4 2
superpathway of phylloquinol biosynthesis 15 1 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 19 3
superpathway of cholesterol biosynthesis 38 4 2
superpathway of chorismate metabolism 59 37 3
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 18 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 10 1