Experiment set1S83 for Acinetobacter baumannii LAC-4

Group: rpmi_tsb_serum temperature
Media: RPMI + 0.1x TSB + Fetal bovine serum (20 vol%)
Culturing: Abaumannii_LAC4_ML3, tube, Aerobic, at 35 (C), shaken=200 rpm
By: Luna lab on 5/28/25
Media components: 10 mg/L Glycine, 200 mg/L L-Arginine, 50 mg/L L-Asparagine, 20 mg/L L-Aspartic Acid, 65 mg/L L-Cysteine hydrochloride monohydrate, 20 mg/L L-Glutamic acid monopotassium salt monohydrate, 300 mg/L L-Glutamine, 15 mg/L L-Histidine, 20 mg/L Hydroxy-L-Proline, 50 mg/L L-Isoleucine, 50 mg/L L-Leucine, 40 mg/L L-Lysine, 15 mg/L L-Methionine, 15 mg/L L-Phenylalanine, 20 mg/L L-Proline, 30 mg/L L-Serine, 20 mg/L L-Threonine, 5 mg/L L-Tryptophan, 29 mg/L L-tyrosine disodium salt, 20 mg/L L-Valine, 0.2 mg/L biotin, 3 mg/L Choline chloride, 0.25 mg/L calcium pantothenate, 1 mg/L Folic Acid, 35 mg/L m-Inositol, 1 mg/L Nicotinamide, 1 mg/L 4-Aminobenzoic acid, 1 mg/L Pyridoxine HCl, 0.2 mg/L Riboflavin, 1 mg/L Thiamine HCl, 0.005 mg/L Cyanocobalamin, 100 mg/L Calcium nitrate tetrahydrate, 48.84 mg/L Magnesium sulfate, 400 mg/L Potassium Chloride, 2000 mg/L Sodium bicarbonate, 6000 mg/L Sodium Chloride, 800 mg/L Sodium phosphate dibasic heptahydrate, 2000 mg/L D-Glucose, 1 mg/L L-glutathione reduced, 5 mg/L Phenol red, 0.1 mg/L Lipopolysaccharide

TSB 0.1x includes: 1.7 g/L Tryptone, 0.3 g/L soytone, 0.25 g/L D-Glucose, 0.5 g/L Sodium Chloride, 0.25 g/L Potassium phosphate dibasic (final concentrations)

Subsystem	#Specific
Copper homeostasis: copper tolerance	1
Phosphate metabolism	1

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