Experiment set1S636 for Mucilaginibacter yixingensis YX-36 DSM 26809

Erythromycin stress 0.05 mg/ml

Group: stress
Media: R2A + Erythromycin (0.05 mg/ml)
Culturing: Mucilaginibacter_YX36_ML5a, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta Torres on 4/20/23
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate

Specific Phenotypes

For 17 genes in this experiment

For stress Erythromycin in Mucilaginibacter yixingensis YX-36 DSM 26809

For stress Erythromycin across organisms

SEED Subsystems

Subsystem	#Specific
Methionine Biosynthesis	2
Methionine Degradation	2
Cobalt-zinc-cadmium resistance	1
Cysteine Biosynthesis	1
Fatty Acid Biosynthesis FASII	1
Glycine and Serine Utilization	1
YeiH	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• Ascorbate and aldarate metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Cysteine metabolism
• Tyrosine metabolism
• Benzoate degradation via hydroxylation
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Selenoamino acid metabolism
• Nucleotide sugars metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Nitrogen metabolism
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-cysteine degradation II	3	3	3
L-serine degradation	3	3	2
D-serine degradation	3	2	2
L-tryptophan degradation II (via pyruvate)	3	2	2
L-methionine degradation II	3	2	2
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast)	12	12	6
gondoate biosynthesis (anaerobic)	4	4	2
L-cysteine biosynthesis III (from L-homocysteine)	2	2	1
oleate biosynthesis IV (anaerobic)	14	13	7
(5Z)-dodecenoate biosynthesis I	6	5	3
palmitate biosynthesis III	29	28	13
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	8	4
tetradecanoate biosynthesis (mitochondria)	25	23	11
superpathway of fatty acid biosynthesis I (E. coli)	16	14	7
palmitate biosynthesis II (type II fatty acid synthase)	31	29	13
fatty acid elongation -- saturated	5	5	2
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	17	8
superpathway of fatty acid biosynthesis initiation	5	4	2
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis	5	2	2
superpathway of fatty acids biosynthesis (E. coli)	53	48	21
superpathway of fatty acid biosynthesis II (plant)	43	39	16
8-amino-7-oxononanoate biosynthesis I	11	9	4
fatty acid biosynthesis initiation (type II)	3	3	1
stearate biosynthesis II (bacteria and plants)	6	5	2
L-methionine biosynthesis II	6	5	2
stearate biosynthesis IV	6	4	2
(5Z)-dodecenoate biosynthesis II	6	4	2
L-threonine degradation I	6	3	2
streptorubin B biosynthesis	34	20	10
L-isoleucine biosynthesis I (from threonine)	7	7	2
glycine betaine degradation III	7	4	2
biotin biosynthesis I	15	13	4
homocysteine and cysteine interconversion	4	3	1
L-mimosine degradation	8	4	2
glycine betaine degradation I	8	4	2
fatty acid biosynthesis initiation (mitochondria)	4	2	1
glutathione-mediated detoxification I	8	3	2
superpathway of L-cysteine biosynthesis (mammalian)	5	4	1
8-amino-7-oxononanoate biosynthesis IV	5	4	1
cis-vaccenate biosynthesis	5	4	1
even iso-branched-chain fatty acid biosynthesis	34	30	6
odd iso-branched-chain fatty acid biosynthesis	34	30	6
anteiso-branched-chain fatty acid biosynthesis	34	30	6
superpathway of L-cysteine biosynthesis (fungi)	6	5	1
hydrogen sulfide biosynthesis II (mammalian)	6	4	1
superpathway of L-isoleucine biosynthesis I	13	13	2
L-cysteine biosynthesis VI (reverse transsulfuration)	7	3	1
hypoglycin biosynthesis	14	3	2
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
2-allylmalonyl-CoA biosynthesis	8	2	1
superpathway of branched chain amino acid biosynthesis	17	17	2
superpathway of L-threonine metabolism	18	11	2
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	9	1
purine nucleobases degradation II (anaerobic)	24	10	2
superpathway of L-methionine salvage and degradation	16	9	1
mycolate biosynthesis	205	18	8
superpathway of mycolate biosynthesis	239	19	8