Experiment set1S593 for Mucilaginibacter yixingensis YX-36 DSM 26809
D-Fructose carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + D-Fructose (20 mM)
Culturing: Mucilaginibacter_YX36_ML5a, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta Torres on 4/20/23
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 3 genes in this experiment
For carbon source D-Fructose in Mucilaginibacter yixingensis YX-36 DSM 26809
For carbon source D-Fructose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-sorbitol degradation I | 3 | 3 | 1 |
| choline-O-sulfate degradation | 3 | 2 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 1 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| sucrose degradation III (sucrose invertase) | 4 | 3 | 1 |
| sucrose degradation VII (sucrose 3-dehydrogenase) | 4 | 2 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 3 | 1 |
| mannitol cycle | 5 | 2 | 1 |
| heterolactic fermentation | 18 | 13 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 15 | 1 |