Experiment set1S538 for Paraburkholderia sabiae LMG 24235

L-Leucine carbon source

Group: carbon source
Media: AB_minimal medium + L-Leucine (20 mM)
Culturing: Psabiae_ML3b, tube, Aerobic, at 28 (C), shaken=180 rpm
By: Marta Torres on 12/5/24

Specific Phenotypes

For 6 genes in this experiment

For carbon source L-Leucine in Paraburkholderia sabiae LMG 24235

For carbon source L-Leucine across organisms

SEED Subsystems

Subsystem	#Specific
Serine-glyoxylate cycle	2
Aromatic amino acid interconversions with aryl acids	1
Cysteine Biosynthesis	1
DNA-binding regulatory proteins, strays	1
HMG CoA Synthesis	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Leucine Degradation and HMG-CoA Metabolism	1
Valine degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism
• Propanoate metabolism
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Tryptophan metabolism
• alpha-Linolenic acid metabolism
• Glyoxylate and dicarboxylate metabolism
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Limonene and pinene degradation
• Caprolactam degradation
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
benzoyl-CoA biosynthesis	3	3	1
2-methyl-branched fatty acid β-oxidation	14	9	3
adipate degradation	5	5	1
fatty acid β-oxidation IV (unsaturated, even number)	5	4	1
adipate biosynthesis	5	4	1
propanoyl-CoA degradation II	5	3	1
fatty acid β-oxidation II (plant peroxisome)	5	3	1
acrylate degradation I	5	3	1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative)	5	1	1
(8E,10E)-dodeca-8,10-dienol biosynthesis	11	6	2
glyoxylate cycle	6	6	1
L-leucine degradation I	6	5	1
β-alanine biosynthesis II	6	4	1
methyl ketone biosynthesis (engineered)	6	3	1
superpathway of glyoxylate cycle and fatty acid degradation	14	11	2
benzoyl-CoA degradation I (aerobic)	7	5	1
fatty acid β-oxidation I (generic)	7	5	1
fatty acid β-oxidation VI (mammalian peroxisome)	7	4	1
L-valine degradation I	8	5	1
phenylacetate degradation I (aerobic)	9	7	1
valproate β-oxidation	9	5	1
benzoate biosynthesis I (CoA-dependent, β-oxidative)	9	5	1
superpathway of coenzyme A biosynthesis II (plants)	10	8	1
3-phenylpropanoate degradation	10	6	1
superpathway of phenylethylamine degradation	11	8	1
Spodoptera littoralis pheromone biosynthesis	22	4	2
oleate β-oxidation	35	28	3
superpathway of glyoxylate bypass and TCA	12	10	1
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase)	13	2	1
docosahexaenoate biosynthesis III (6-desaturase, mammals)	14	2	1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	22	1
platensimycin biosynthesis	26	6	1