Experiment set1S538 for Alteromonas macleodii MIT1002

Mixed culture with Prochlorococcus marinus str. MIT9313; Experiment A, time point 5

Group: mixed community
Media: Pro99_seawater + Mixed culture; Prochlorococcus marinus str. MIT9313 10:1 starting OD to HP15
Culturing: Alteromonas_MIT1002_ML10, bottle_100mL, Aerobic, at 24 (C), shaken=0 rpm
By: Christine Ziegler on 12/1/24
Media components: 50 uM Sodium phosphate monobasic monohydrate, 800 uM Ammonium chloride, 1.17 uM EDTA (disodium salt), 1.18 uM Ferric chloride, 0.008 uM Zinc sulfate heptahydrate, 0.005 uM Cobalt chloride hexahydrate, 0.09 uM Manganese (II) chloride tetrahydrate, 0.003 uM Sodium Molybdate Dihydrate, 0.01 uM Sodium selenite pentahydrate, 0.01 uM Nickel (II) chloride hexahydrate, 1 X Filtered Seawater

Specific Phenotypes

For 2 genes in this experiment

For mixed community Mixed culture; Prochlorococcus marinus str. MIT9313 10:1 starting OD to HP15 in Alteromonas macleodii MIT1002

For mixed community Mixed culture; Prochlorococcus marinus str. MIT9313 10:1 starting OD to HP15 across organisms

SEED Subsystems

Subsystem	#Specific
Catechol branch of beta-ketoadipate pathway	1
Leucine Degradation and HMG-CoA Metabolism	1
Protocatechuate branch of beta-ketoadipate pathway	1
Serine-glyoxylate cycle	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• 1- and 2-Methylnaphthalene degradation
• Butanoate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ketolysis	3	3	1
TCA cycle VI (Helicobacter)	9	6	1