Experiment set1S478 for Escherichia coli ECRC62
RB68
Group: phageMedia: LB_plus_SM_buffer + MOI 10
Culturing: Ecoli_ECRC62_ML1, 48 well microplate, Aerobic, at 37 (C), shaken=double orbital, continuous, 205cpm
By: Lucas on 11/24/2024
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride, 100 mM Sodium Chloride, 4 mM Magnesium sulfate, 0.005 vol% Gelatin, 25 mM Tris hydrochloride
Specific Phenotypes
For 4 genes in this experiment
For phage MOI 10 in Escherichia coli ECRC62
For phage MOI 10 across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Pentose phosphate pathway
- Nicotinate and nicotinamide metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| ribose phosphorylation | 2 | 2 | 1 |
| 2-deoxy-D-ribose degradation I | 3 | 3 | 1 |
| queuosine biosynthesis I (de novo) | 4 | 4 | 1 |
| NAD salvage pathway V (PNC V cycle) | 5 | 3 | 1 |
| queuosine biosynthesis III (queuosine salvage) | 5 | 3 | 1 |
| NAD salvage pathway I (PNC VI cycle) | 7 | 5 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of NAD biosynthesis in eukaryotes | 14 | 7 | 1 |