Experiment set1S466 for Bacteroides stercoris CC31F

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Mucin from porcine stomach type III 2.5 mg/ml carbon source

Group: carbon source
Media: Varel_Bryant_medium + Mucin from porcine stomach type III (2.5 mg/mL)
Culturing: Bstercoris_CC31F_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 10/31/24
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source Mucin from porcine stomach type III in Bacteroides stercoris CC31F

For carbon source Mucin from porcine stomach type III across organisms

SEED Subsystems

Subsystem #Specific
Coenzyme B12 biosynthesis 1
D-Galacturonate and D-Glucuronate Utilization 1
Deoxyribose and Deoxynucleoside Catabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
2-deoxy-D-ribose degradation I 3 2 1
2-deoxy-α-D-ribose 1-phosphate degradation 3 1 1
4-deoxy-L-threo-hex-4-enopyranuronate degradation 5 5 1
superpathway of pyrimidine deoxyribonucleosides degradation 6 3 1
superpathway of purine deoxyribonucleosides degradation 7 4 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 15 1