Experiment set1S448 for Phocaeicola dorei CL03T12C01

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N-acetylneuraminic acid carbon source

Group: carbon source
Media: Varel_Bryant_medium + N-acetylneuraminic acid (5 mM)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya on 10/1/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 10 genes in this experiment

For carbon source N-acetylneuraminic acid in Phocaeicola dorei CL03T12C01

For carbon source N-acetylneuraminic acid across organisms

SEED Subsystems

Subsystem #Specific
Sialic Acid Metabolism 3
Fermentations: Lactate 1
Fermentations: Mixed acid 1
Galactosylceramide and Sulfatide metabolism 1
Iron acquisition in Vibrio 1
Ton and Tol transport systems 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to (R)-lactate 1 1 1
N-acetylneuraminate and N-acetylmannosamine degradation II 3 2 1
vancomycin resistance I 3 2 1
L-alanine degradation II (to D-lactate) 3 2 1
L-alanine degradation VI (reductive Stickland reaction) 6 1 1
lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) 8 4 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 7 1
superpathway of L-alanine fermentation (Stickland reaction) 9 4 1
mixed acid fermentation 16 11 1
superpathway of glucose and xylose degradation 17 16 1
heterolactic fermentation 18 14 1