Experiment set1S447 for Phocaeicola dorei CL03T12C01

N-acetylneuraminic acid carbon source

Group: carbon source
Media: Varel_Bryant_medium + N-acetylneuraminic acid (5 mM)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya on 10/1/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 9 genes in this experiment

For carbon source N-acetylneuraminic acid in Phocaeicola dorei CL03T12C01

For carbon source N-acetylneuraminic acid across organisms

SEED Subsystems

Subsystem	#Specific
Sialic Acid Metabolism	3
Galactosylceramide and Sulfatide metabolism	1
Iron acquisition in Vibrio	1
Ton and Tol transport systems	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Other glycan degradation
• Nucleotide sugars metabolism
• Aminosugars metabolism
• Sphingolipid metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
N-acetylneuraminate and N-acetylmannosamine degradation II	3	2	1