Experiment set1S411 for Phocaeicola dorei CL03T12C01

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D-Mannose carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Mannose (20 mM)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya on 10/1/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 6 genes in this experiment

For carbon source D-Mannose in Phocaeicola dorei CL03T12C01

For carbon source D-Mannose across organisms

SEED Subsystems

Subsystem #Specific
Coenzyme B12 biosynthesis 1
Glycolysis and Gluconeogenesis 1
Potassium homeostasis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
sucrose biosynthesis I (from photosynthesis) 9 6 1
glycolysis II (from fructose 6-phosphate) 11 9 1
gluconeogenesis III 12 9 1
formaldehyde assimilation III (dihydroxyacetone cycle) 12 9 1
gluconeogenesis I 13 10 1
glycolysis I (from glucose 6-phosphate) 13 10 1
Calvin-Benson-Bassham cycle 13 8 1
superpathway of glycolysis and the Entner-Doudoroff pathway 17 13 1
oxygenic photosynthesis 17 8 1
superpathway of hexitol degradation (bacteria) 18 11 1
superpathway of anaerobic sucrose degradation 19 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 13 1
superpathway of N-acetylneuraminate degradation 22 16 1
ethene biosynthesis V (engineered) 25 14 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 17 1
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 12 1
1-butanol autotrophic biosynthesis (engineered) 27 13 1