Experiment set1S401 for Phocaeicola dorei CL03T12C01

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B-glucan from yeast carbon source

Group: carbon source
Media: Varel_Bryant_medium + B-glucan from yeast (5 mg/mL)
Culturing: Bdorei_CL03T12C01_ML9, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 34 (C), shaken=0 rpm
By: Surya on 10/1/23
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 2 genes in this experiment

For carbon source B-glucan from yeast in Phocaeicola dorei CL03T12C01

For carbon source B-glucan from yeast across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
long-chain fatty acid activation 1 1 1
γ-linolenate biosynthesis II (animals) 2 1 1
linoleate biosynthesis II (animals) 2 1 1
3-methyl-branched fatty acid α-oxidation 6 2 2
alkane biosynthesis II 3 1 1
oleate biosynthesis I (plants) 3 1 1
phytol degradation 4 2 1
wax esters biosynthesis II 4 1 1
phosphatidylcholine acyl editing 4 1 1
long chain fatty acid ester synthesis (engineered) 4 1 1
sporopollenin precursors biosynthesis 18 4 4
sphingosine and sphingosine-1-phosphate metabolism 10 2 2
octane oxidation 5 1 1
stearate biosynthesis II (bacteria and plants) 6 5 1
stearate biosynthesis IV 6 4 1
fatty acid salvage 6 2 1
6-gingerol analog biosynthesis (engineered) 6 1 1
stearate biosynthesis I (animals) 6 1 1
icosapentaenoate biosynthesis III (8-desaturase, mammals) 7 1 1
ceramide degradation by α-oxidation 7 1 1
arachidonate biosynthesis III (6-desaturase, mammals) 7 1 1
capsaicin biosynthesis 7 1 1
icosapentaenoate biosynthesis II (6-desaturase, mammals) 7 1 1
ceramide and sphingolipid recycling and degradation (yeast) 16 2 2
suberin monomers biosynthesis 20 2 2
superpathway of fatty acid biosynthesis II (plant) 43 37 4
palmitate biosynthesis II (type II fatty acid synthase) 31 29 2
cutin biosynthesis 16 1 1
superpathway of fatty acids biosynthesis (E. coli) 53 47 2
palmitate biosynthesis III 29 21 1
oleate β-oxidation 35 4 1